Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Abnormal Proliferation

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Targeting the ATX-LPA Axis Overcomes TKI Resistance and Immunosuppression in Renal Cell Carcinoma via Dual Inhibition of AKT/mTOR and TBK1/IRF3 Pathways.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Correction to "Microchip-Based Structure Determination of Disease-Relevant p53".

Analytical chemistry·2026

Same author

Oral connexin43-peptide-loaded milk extracellular vesicles mitigate lethal radiation injury while preserving tumour radiosensitivity.

Cancer letters·2026

Same author

Reversing methanogenic archaea to mitigate anthropogenic methane emissions and generate value-added products.

Trends in biotechnology·2026

Same author

Integrative transcriptomics and metabolomics analyses reveal changes in meat quality and muscle lipid metabolism in sheep supplemented with rumen-protected glucose.

Meat science·2026

Same author

Recurrent SARS-CoV-2 Omicron broadly neutralizing humanized antibodies in different single human V<sub>H</sub>1-2-rearranging mouse models.

Proceedings of the National Academy of Sciences of the United States of America·2026

Search research articles

Related Experiment Video

Updated: Dec 3, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

Microchip-Based Structure Determination of Disease-Relevant p53.

Maria J Solares^1,2,3, G M Jonaid^4,2,3, William Y Luqiu^3,5

¹Molecular, Cellular, and Integrative Biosciences Graduate Program, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania 16802, United States.

Analytical Chemistry

|October 30, 2020

Summary

Researchers developed a microchip technology to study the tumor suppressor protein TP53 (p53) structure. This method reveals new details about p53 assemblies and ubiquitination sites, crucial for understanding cancer progression.

More Related Videos

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Related Experiment Videos

Last Updated: Dec 3, 2025

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

High Sensitivity Measurement of Transcription Factor-DNA Binding Affinities by Competitive Titration Using Fluorescence Microscopy

Published on: February 7, 2019

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Counting Proteins in Single Cells with Addressable Droplet Microarrays

Published on: July 6, 2018

Area of Science:

Biochemistry
Molecular Biology
Nanotechnology

Background:

The tumor suppressor protein TP53 (p53) is vital in human cells, and its mutations are linked to cancer.
Incomplete structural information for p53 hinders a full understanding of its function and role in disease.
Existing methods for p53 structural analysis face limitations.

Purpose of the Study:

To present a novel microchip-based technology for studying p53 assemblies.
To obtain high-resolution structural data of p53 from human cancer cells.
To investigate the structural basis of p53's role in cancer.

Main Methods:

Development of a microchip device for p53 structural studies without altering the native gene sequence or post-translational modifications.
Application of cryo-electron microscopy (cryo-EM) for structural determination.
Analysis of p53 monomer and tetramer structures.

Main Results:

Cryo-EM resolved structures of the p53 monomer at ~4.8 Å and tetramer at ~7 Å.
The structures provided insights into flexible regions of p53.
Biologically relevant ubiquitination sites on p53 were identified.

Conclusions:

The microchip technology offers a new platform for p53 structural studies.
The resolved structures enhance understanding of p53's molecular mechanisms.
This approach provides a framework for investigating the oncogenic impact of p53.