Tissue-scale tensional homeostasis in skin regulates structure and physiological function

Area of Science:

• Biomedical Engineering
• Tissue Engineering
• Dermatology

Background:

• Tensional homeostasis is essential for organ and tissue development, influencing morphological and functional properties.
• Skin's integrity, flexibility, and protective functions rely on internal tension-distribution patterns involving cells and extracellular matrices (ECMs).

Purpose of the Study:

• To investigate the impact of horizontal tension-force balance on a human skin equivalent (HSE).
• To explore how tension influences skin homeostasis and the activation of mechanosensitive molecules.

Main Methods:

• Utilized a human skin equivalent (HSE) model.
• Applied horizontal tension to the dermal layer of the HSE.
• Analyzed changes in HSE characteristics and molecular activation.

Main Results:

• Horizontal tension significantly improved HSE characteristics, including cell-ECM physical relationships.
• Tension promoted skin homeostasis via activation of mechanosensitive molecules like ROCK and MRTF-A.
• Results showed favorable comparisons to tension-released models.

Conclusions:

• Horizontal tension-force balance enhances human skin equivalent properties.
• Tension-induced HSE serves as a valuable alternative model for studying skin physiological functions regulated by tensional homeostasis.
• This research provides insights into the role of mechanical forces in skin development and maintenance.