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Design of Affinity Chromatography Peptide Ligands Through Combinatorial Peptide Library Screening
G R Barredo1,2, S L Saavedra1,2, M C Martínez-Ceron1,2
1Cátedra de Biotecnología. Facultad de Farmacia y Bioquímica,, Universidad de Buenos Aires, Buenos Aires, Argentina.
Abstract:
In this chapter, a protocol to design affinity chromatography matrices with short peptide ligands immobilized for protein purification is described. The first step consists of the synthesis of a combinatorial peptide library on the hydroxymethylbenzoyl (HMBA)-ChemMatrix resin by the divide-couple-recombine (DCR) method using the Fmoc chemistry. Next, the library is screened with the protein of interest labeled with a fluorescent dye or biotin. Subsequently, peptides contained on positive beads are identified by tandem matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS/MS), and those sequences showing greater consensus are synthesized in larger quantities and immobilized on chromatographic supports. Finally, target protein adsorption on peptide affinity matrices is evaluated through equilibrium adsorption isotherms and breakthrough curves.
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