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Related Experiment Video

Updated: Dec 3, 2025

High Throughput MicroRNA Profiling: Optimized Multiplex qRT-PCR at Nanoliter Scale on the Fluidigm Dynamic ArrayTM IFCs
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Target-triggered entropy-driven amplification system-templated silver nanoclusters for multiplexed microRNA analysis.

Fengyun Li1, Gen Li1, Shijie Cao2

  • 1School of Chinese Materia Medica, State Key Laboratory of Component-based Chinese Medicine, Tianjin University of Traditional Chinese Medicine, 10 Poyanghu Road, Tianjin, 301617, PR China.

Biosensors & Bioelectronics
|October 31, 2020
PubMed
Summary

This study introduces a new sensing platform for detecting multiple tumor-associated microRNAs (miRNAs) simultaneously. The novel system utilizes entropy-driven amplification and silver nanoclusters for sensitive and selective cancer biomarker analysis.

Keywords:
Biomarker analysisEntropy-driven catalysisMultiplexed detectionSilver nanoclustersmicroRNA

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Area of Science:

  • Biomarker Discovery
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • MicroRNAs (miRNAs) are crucial biomarkers for disease diagnosis, prognosis, and treatment.
  • Simultaneous detection of multiple miRNAs is essential for early and accurate cancer diagnosis.
  • Existing methods for miRNA detection require improvement in sensitivity and selectivity.

Purpose of the Study:

  • To develop a novel sensing platform for the multiplexed analysis of tumor-associated miRNAs.
  • To couple target-triggered entropy-driven catalysis with luminescence-adjustable DNA-templated silver nanoclusters (Ag NCs).
  • To achieve sensitive and selective detection of multiple miRNAs for cancer diagnostics.

Main Methods:

  • Development of an entropy-driven amplification system-templated silver nanoclusters (Ag NCs) sensing platform.
  • Utilizing target-triggered entropy-driven catalysis and luminescence-adjustable DNA-templated Ag NCs.
  • Constructing yellow-emitting and red-emitting Ag NCs biosensors for specific miRNA detection.

Main Results:

  • The sensing platform achieved sensitive detection of miRNA-141 (6.1 pM) and miRNA-155 (8.7 pM).
  • Multiplexed synchronous detection of miRNA-141 and miRNA-155 was successfully demonstrated.
  • The platform showed excellent selectivity, flexibility, and narrow-band excitation properties.

Conclusions:

  • The developed sensing platform provides a powerful tool for multiplexed biomarker analysis.
  • This strategy offers a simple, flexible, and convenient approach for clinical applications.
  • The platform facilitates early and accurate diagnosis of cancers through sensitive miRNA detection.