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Enhanced low-temperature denitrification by microbial consortium using solid-phase humin.

Zhixing Xiao1, Lei Zhang2, Zhi-Ling Li3

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|November 1, 2020
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Summary

Humin, a natural solid, enhances groundwater denitrification by acting as an electron donor after acetate depletion. This sustainable approach boosts microbial diversity for effective nitrate removal.

Keywords:
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Area of Science:

  • Environmental Microbiology
  • Bioremediation Science

Background:

  • Biostimulation of heterotrophic denitrification often relies on liquid organic carbon electron donors, posing sustainability challenges.
  • Solid-phase humin offers a cost-effective, natural alternative for enhancing denitrification.

Purpose of the Study:

  • To investigate the efficacy of humin as an electron donor for low-temperature denitrification.
  • To assess humin's impact on microbial community structure and function during denitrification.

Main Methods:

  • Incubation of an acetate-fed microbial community with humin at 20°C and 10°C.
  • Analysis of acetate consumption, bacterial growth, electron balance, and first-order kinetics.
  • Assessment of denitrifying microbial community richness and diversity.

Main Results:

  • Humin addition enhanced low-temperature denitrification capacity, particularly after acetate exhaustion.
  • Humin acted as an additional electron donor, likely via its reduced quinone groups.
  • Humin increased the richness and diversity of the denitrifying microbial community, with Dechloromonas spp. being key players.

Conclusions:

  • Humin serves as a viable solid-phase electron donor for sustainable groundwater denitrification.
  • This approach reduces reliance on liquid organic carbon sources.
  • The findings have significant implications for bioremediation of nitrate-contaminated groundwater.