3D cell-printing of biocompatible and functional meniscus constructs using meniscus-derived bioink

Area of Science:

• Biomaterials Science
• Tissue Engineering
• Orthopedic Surgery

Background:

• Meniscus injuries are common and challenging to treat due to the tissue's complex structure.
• Current treatments often fail to fully restore meniscus function, highlighting the need for advanced regenerative strategies.

Purpose of the Study:

• To develop and evaluate 3D cell-printed meniscus constructs for enhanced tissue regeneration.
• To assess the biocompatibility and mechanical properties of these constructs in vivo.

Main Methods:

• Fabrication of 3D meniscus constructs using a blend of polyurethane, polycaprolactone, and cell-laden decellularized meniscal extracellular matrix (me-dECM) bioink.
• Evaluation of the bioink's ability to support stem cell proliferation and differentiation.
• In vivo assessment of the constructs' biocompatibility, mechanical performance, and biological function.

Main Results:

• The me-dECM bioink supported stem cell growth and directed differentiation towards fibrochondrocytes.
• 3D cell-printed constructs demonstrated excellent biocompatibility and mechanical properties comparable to native meniscus tissue.
• In vivo studies confirmed improved biological functionality of the engineered meniscus constructs.

Conclusions:

• 3D cell-printing technology combined with me-dECM bioink effectively replicates meniscus tissue-specific shape and microenvironment.
• The developed meniscus constructs hold significant potential for clinical applications in meniscus regeneration.