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Should nagarse be used during the isolation of brain mitochondria?
1Department of Clinical Neurological Sciences, University of Western Ontario, Victoria Hospital, London, Canada.
Neurochemical Research
|September 1, 1987
Abstract:
When nagarse is used to isolate brain mitochondria, a proportion of the nagarse stays associated with the mitochondrial fraction. This results in no detrimental affect on the respiratory activities. The nagarse is active in the presence of 2.3% sodium dodecyl sulfate and when samples are prepared for sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the nagarse degrades a substantial amount of the mitochondrial proteins.