Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

[Endothelial function and arteriosclerosis].

R P Franke1, S Höpken, H Schnittler

  • 1Abt. Pathologie, Klinikum der RWTH Aachen.

Onkologie
|August 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

[Protein Z deficiency in unexplained affinity to thromboses, bleedings or abortions].

Hamostaseologie·2012
Same author

[Risk pregnancies and how to treat them].

Hamostaseologie·2012
Same author

Influence of blood donation on levels of water-soluble vitamins.

Transfusion medicine (Oxford, England)·2009
Same author

Experiences with recombinant FVIIa in the emergency treatment of patients with autoimmune thrombocytopenia: a review of the literature.

Annals of hematology·2008
Same author

Influence of peptidylarginine deiminase type 4 genotype and shared epitope on clinical characteristics and autoantibody profile of rheumatoid arthritis.

Annals of the rheumatic diseases·2008
Same author

Viral haemorrhagic fever and vascular alterations.

Hamostaseologie·2008
Same journal

Successful treatment with nab-paclitaxel and gemcitabine after FOLFIRINOX failure in a patient with metastasized pancreatic adenocarcinoma.

Onkologie·2013
Same journal

Metastatic germ cell tumour following renal transplantation.

Onkologie·2013
Same journal

miR-145 inhibits proliferation and invasion of esophageal squamous cell carcinoma in part by targeting c-Myc.

Onkologie·2013
Same journal

A meta-analysis of internal mammary lymph node metastasis in breast cancer patients.

Onkologie·2013
Same journal

Patient-related delay in presentation for cutaneous squamous cell carcinoma. A cross-sectional clinical study.

Onkologie·2013
Same journal

Treatment of primary breast cancer at the surgical unit of the Charité 1984-1998.

Onkologie·2013
See all related articles

High levels of low-density lipoprotein (LDL) damage endothelial cells. Shear stress increases LDL uptake in these cells, indicating potential dysfunction in blood filtration.

Area of Science:

  • Endothelial cell biology
  • Cardiovascular research
  • Lipid metabolism

Context:

  • Elevated plasma low-density lipoprotein (LDL) is linked to endothelial damage and impaired antithrombogenicity.
  • Endothelial cell dysfunction may negatively affect blood component separation from subendothelial tissues.

Purpose:

  • To investigate endothelial dysfunction in relation to LDL transport under physiological flow conditions.
  • To quantify LDL accumulation in human umbilical venous endothelial cells (HUVEC) under varying shear stress levels.

Summary:

  • Human umbilical venous endothelial cells (HUVEC) were incubated with LDL (100 µg/ml) and subjected to physiological shear stress (0.5 and 2.5 dyn/cm²).
  • Oil-red-O staining quantified LDL uptake, revealing minimal staining without shear stress.

Related Experiment Videos

  • Shear stress significantly increased LDL accumulation: 1.3% (orthogonal) and 0.3% (cross-sectional) at 0.5 dyn/cm², and 4.6% (orthogonal) and 8.7% (cross-sectional) at 2.5 dyn/cm².

    • Impact:

    • Demonstrates that physiological shear stress enhances LDL uptake in HUVEC, contributing to understanding endothelial dysfunction.
    • Provides quantitative data on LDL accumulation influenced by shear stress, relevant for cardiovascular disease research.
    • Highlights the role of mechanical forces in modulating lipid transport across the endothelium.