[Establishment and Validation of Animal Models of Peripheral Diabetic Neuropathies]

Objective To analyze the common animals,modeling methods,modeling indicators,and applicable indicators of animal models of type 2 diabetic peripheral neuropathies(DPN)by literature review.Methods Literature on animal experiments concerning type 2 DPN were searched in databases including CNKI,PubMed,and Wanfang Database.The relevant indicators and data were statistically analyzed.Results SD rats were commonly used as the modeling animals of type 2 DPN,and the commonly used method was experimental induction,during which a combination of low-dose streptozotocin(STZ)and high-energy intake was applied.Indicators of successful modeling of type 2 diabetes mellitus included:fasting blood glucose>11.1 mmol/L or random blood glucose> 13.8 mmol/L and insulin resistance;indicators of successful DPN modeling included:decreased nerve conduction velocity,abnormal thermal and mechanical pain,abnormal morphology of sciatic nerve,and decreased subepidermal nerve fibers.Conclusions Type 2 DPN animal modeling usually uses SD male rats,which are fed with high-sugar and high-fat diet(containing 10% lard and 20% sucrose)or high-fat diet(containing 45%-60% Lard)combined with a single intraperitoneal injection of low-dose STZ(25-35 mg/kg),which is featured by high modeling rate,low mortality,and strong operability.With the success of modeling can be judged by the behaviors,pathomorphology,and neuroelectricity of the animals.These findings may inform further studies on the mechanisms of early-stage type 2 DPN and its interventions.