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Updated: Dec 2, 2025

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Combining X-Ray Crystallography with Small Angle X-Ray Scattering to Model Unstructured Regions of Nsa1 from S. Cerevisiae

Published on: January 10, 2018

Combined Analysis Based on a Crystalline Sponge Method.

Kazuaki Ohara¹, Kentaro Yamaguchi¹

¹Faculty of Pharmaceutical Sciences at Kagawa Campus, Tokushima Bunri University, 1314-1 Shido, Sanuki, Kagawa, 769-2193, Japan.

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry

|November 2, 2020

Summary

This summary is machine-generated.

The crystalline sponge (CS) method enables X-ray crystallographic analysis without analyte crystallization. This technique determines the absolute structure of trace analytes, revolutionizing microanalysis.

Keywords:

Crystalline sponge X-ray crystallography laser desorption ionization mass spectrometry structure determination

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$Microcrystallography of Protein Crystals and In Cellulo Diffraction$

Microcrystallography of Protein Crystals and In Cellulo Diffraction

Published on: July 21, 2017

Area of Science:

Analytical Chemistry
Structural Biology
Materials Science

Background:

Traditional X-ray crystallography requires analyte crystallization, a significant hurdle for many samples.
The crystalline sponge (CS) method offers an alternative approach for molecular structure determination.
CS possesses unique molecular recognition properties and a flexible framework for accommodating analytes.

Purpose of the Study:

To review the discovery and applications of the crystalline sponge (CS) method.
To highlight CS's utility in analyzing trace analytes and determining absolute molecular structures.
To showcase CS in conjunction with NMR and mass spectrometry for microanalysis.

Main Methods:

X-ray crystallography using crystalline sponges.
Nuclear Magnetic Resonance (NMR) spectroscopy.
Mass Spectrometry (MS) coupled with CS.

Main Results:

The CS method allows for molecular structure analysis without prior crystallization.
Sub-milligram amounts of analyte are sufficient for structure determination.
Combined CS-NMR and CS-MS approaches demonstrate effective microanalysis of unknown samples.

Conclusions:

The crystalline sponge method is a powerful tool for determining the absolute structure of trace analytes.
CS, especially when combined with spectroscopic techniques, offers significant potential for microanalysis.
Emerging applications include using crystalline nano-surfaces for detecting small metabolites and post-translational biomolecules.