Activated Human Memory B Lymphocytes Use CR4 (CD11c/CD18) for Adhesion, Migration, and Proliferation
Zsuzsa Nagy-Baló1, Richárd Kiss2, Alina Menge1
1Department of Immunology, Eötvös Loránd University, Budapest, Hungary.
Frontiers in Immunology
|November 2, 2020
Summary
Complement receptor 4 (CR4) is newly synthesized on activated human B cells, enhancing their adhesion, migration, and proliferation, thus playing a functional role in memory B cell responses.
Area of Science:
- Immunology
- Cell Biology
Background:
- Complement receptors CR3 and CR4 (β2-integrins) are crucial for myeloid cell functions.
- The role of these integrins in human B lymphocytes remains underexplored, though CD11c+ B cells are linked to memory cells.
Purpose of the Study:
- To investigate the expression and function of complement receptors CR3 and CR4 on human B lymphocytes.
- To determine the role of CD11c on activated B cells in memory cell responses.
Main Methods:
- Isolation and stimulation of human B cells from tonsils and peripheral blood.
- Flow cytometry to analyze CD11c and CD11b expression.
- Assessment of B cell adhesion, migration, and proliferation.
Main Results:
- CD11c expression significantly increased on B cells after B cell receptor (BCR) stimulation, while CD11b remained low.
- CD11c+ B cells were identified as memory cells, with expression correlating with class switching.
- CD11c mediated adhesion promoted proliferation in BCR-activated B cells.
Conclusions:
- CD11c is functionally expressed on BCR-activated human B cells, contributing to adhesion, migration, and proliferation.
- These findings highlight CD11c as a functional driver of memory B cell responses, not just a marker.
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