Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

15.1K
The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
In...
15.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Vaccination generates broadly cross-neutralizing antibodies to the HIV Env apex.

Nature·2026
Same author

Structural and immunogenetic signatures guide CD4-mimetic HIV vaccine development.

Cell reports·2026
Same author

Genetically diverse influenza antibodies highlight the role of IG germline gene variation and inform population-comprehensive vaccine strategies.

Immunity·2026
Same author

Ultra-high-throughput IGH genotyping of 25 global populations reveals population-biased allelic diversity and homozygous V and D gene deletions.

Immunity·2026
Same author

The impact of B-cell reconstitution on mRNA vaccine responses in allogeneic stem cell transplant recipients.

Clinical & translational immunology·2026
Same author

Population-level genomic analysis of immunoglobulin loci variation in rhesus macaques reveals extensive germline diversity.

Immunity·2026

Related Experiment Video

Updated: Dec 2, 2025

Colony Forming Cell CFC Assay for Human Hematopoietic Cells
11:30

Colony Forming Cell CFC Assay for Human Hematopoietic Cells

Published on: December 18, 2010

36.2K

Flow Cytometry-Based Protocols for the Analysis of Human Plasma Cell Differentiation.

Sharesta Khoenkhoen1, Monika Ádori1, Gabriel K Pedersen1

  • 1Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Frontiers in Immunology
|November 2, 2020
PubMed
Summary

Researchers developed a new in vitro method to study B cell differentiation into antibody-producing plasma cells (PCs). This technique aids in understanding primary immunodeficiencies and potential defects in immune responses.

Keywords:
B cell proliferationBlimp-1/PRDM1CVIDIRF4Pax5common variable immunodeficiencyplasma cell differentiation

More Related Videos

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
10:58

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

14.3K
Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

17.8K

Related Experiment Videos

Last Updated: Dec 2, 2025

Colony Forming Cell CFC Assay for Human Hematopoietic Cells
11:30

Colony Forming Cell CFC Assay for Human Hematopoietic Cells

Published on: December 18, 2010

36.2K
Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry
10:58

Discrimination of Seven Immune Cell Subsets by Two-fluorochrome Flow Cytometry

Published on: March 5, 2019

14.3K
Flow Cytometric Characterization of Murine B Cell Development
08:25

Flow Cytometric Characterization of Murine B Cell Development

Published on: January 22, 2021

17.8K

Area of Science:

  • Immunology
  • Cell Biology
  • Genetics

Background:

  • Humoral immunity relies on B cells differentiating into antibody-producing plasma cells (PCs).
  • Primary immunodeficiencies often involve B cell defects, leading to hypogammaglobulinemia and infection susceptibility.
  • Common Variable Immunodeficiency (CVID) is a prevalent symptomatic primary immunodeficiency with poorly understood genetic causes.

Purpose of the Study:

  • To develop an in vitro protocol for analyzing human B cell differentiation into PCs and antibody production.
  • To identify key cellular markers and pathways involved in B cell to PC differentiation.
  • To establish a method applicable to studying B cell defects in primary immunodeficiency patients.

Main Methods:

  • Developed an in vitro stimulation protocol for primary human B cells.
  • Utilized flow cytometry to identify specific cell populations (CD38+, IRF4+, Blimp-1+).
  • Assessed antibody production and B cell differentiation, including from cryopreserved samples.

Main Results:

  • Successfully established a protocol for in vitro B cell stimulation and analysis of PC differentiation.
  • Identified a population of CD38+, IRF4+, Blimp-1+ cells committed to PC fate and IgG production.
  • Demonstrated the protocol's efficacy with cryopreserved samples, showing potential for clinical application.

Conclusions:

  • The developed in vitro method enables robust analysis of B cell differentiation into antibody-producing plasma cells.
  • This protocol can be applied to investigate defects in B cell differentiation in patients with primary antibody deficiencies.
  • Further application of these functional assays may elucidate disease pathophysiology and underlying mechanisms in primary immunodeficiencies.