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Rapid identification of Nocardia cyriacigeorgica from a brain abscess patient using MALDI-TOF-MS

Mohammed AlMogbel1, Mohammed AlBolbol2, Noura Elkhizzi3

  • 1Molecular Diagnostic and Personalized Therapeutics unit, College of Applied Medical Sciences, University of Ha'il, Ha'il, Kingdom of Saudi Arabia.

Oxford Medical Case Reports
|November 2, 2020
PubMed

Insights

Nocardia cyriacigeorgica infections are challenging to diagnose. Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) offers a rapid and accurate method for identifying this bacterium in clinical settings.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases

Background:

  • Nocardia cyriacigeorgica is a pathogen often causing severe infections in immunocompromised individuals.
  • Traditional methods for isolating and identifying N. cyriacigeorgica in diagnostic labs are time-consuming and complex.

Observation:

  • A case of Nocardia cyriacigeorgica infection in a 65-year-old female patient is reported.
  • The bacterium was isolated from a patient in a neurology ward in Riyadh, Saudi Arabia.

Findings:

  • Matrix-assisted laser desorption/ionization-time of flight-mass spectrometry (MALDI-TOF-MS) rapidly and accurately identified N. cyriacigeorgica.
  • Species identification was confirmed using 16S ribosomal RNA sequence analysis.

Implications:

  • MALDI-TOF-MS integration can significantly improve the diagnostic workflow for Nocardia infections.
  • This technology aids in the timely identification of difficult-to-identify bacterial species, enhancing patient care.

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