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LncRNA MALAT1 Affects Mycoplasma pneumoniae Pneumonia via NF-κB Regulation

Haiyan Gu1, Yifan Zhu1, Yao Zhou1

  • 1Department of Respiratory Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.

Frontiers in Cell and Developmental Biology
|November 2, 2020
PubMed

Insights

The long non-coding RNA MALAT1 is elevated in Mycoplasma pneumoniae pneumonia (MPP) and drives inflammation by activating the NF-κB pathway. Silencing MALAT1 reduces inflammatory responses in both cell and mouse models of MPP.

Area of Science:

  • Molecular Biology
  • Immunology
  • Respiratory Medicine

Background:

  • Mycoplasma pneumoniae pneumonia (MPP) is a common respiratory infection.
  • The role of long non-coding RNAs (lncRNAs) in MPP pathogenesis is not fully understood.
  • Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) is a lncRNA implicated in various cellular processes.

Purpose of the Study:

  • To investigate the involvement of MALAT1 in MPP.
  • To elucidate the underlying mechanism of MALAT1's action in MPP.
  • To assess the therapeutic potential of targeting MALAT1 in MPP.

Main Methods:

  • Comparison of MALAT1 expression in bronchoalveolar lavage fluid from children with MPP versus controls.
  • In vitro studies using human airway epithelial cells infected with Mycoplasma pneumoniae, involving MALAT1 knockdown.
  • In vivo studies using a mouse model of MP pneumonia with MALAT1 silencing via adenovirus-mediated RNAi.

Main Results:

  • MALAT1 expression was significantly higher in children with MPP, correlating with increased inflammatory mediators IL-8 and TNF-α.
  • MALAT1 knockdown suppressed IL-8 and TNF-α expression and reduced NF-κB activation in vitro.
  • MALAT1 silencing in vivo partially reversed pulmonary inflammation, vascular permeability, and inflammatory factor secretion in MP-infected mice.

Conclusions:

  • MALAT1 plays a significant role in regulating airway and pulmonary inflammation during Mycoplasma pneumoniae infection.
  • The mechanism involves the regulation of the NF-κB signaling pathway.
  • MALAT1 represents a potential therapeutic target for managing MPP.
Keywords:
Mycoplasma pneumoniae pneumoniainflammationlncRNAmetastasis associated lung adenocarcinoma transcript 1nuclear factor-κB

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