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Collagen and Eosinophils in Jenny's Endometrium: Do They Differ With Endometrial Classification?
Jordi Miró1, Miguel Gutiérrez-Reinoso1, Joana Aguiar da Silva2
1Equine Reproduction Service, Department of Animal Medicine and Surgery, Faculty of Veterinary Medicine, Autonomous University of Barcelona, Cerdanyola del Vallès, Spain.
Frontiers in Veterinary Science
|November 2, 2020
Summary
In jenny endometrium, decreasing eosinophils and neutrophils correlate with increased fibrosis. Interleukin-33 (IL33) transcripts rise with fibrosis, suggesting a role in chronic changes and potential fibroblast involvement.
Area of Science:
- Veterinary Science
- Reproductive Biology
- Histopathology
Background:
- The Kenney and Doig classification for mare endometrium relies on collagen fibers and inflammatory cells.
- Eosinophil infiltration in jenny endometrium is notable, with both eosinophils and fibroblasts producing IL33, a cytokine linked to fibrosis.
- Understanding endometrial changes in jennies is crucial for reproductive health.
Purpose of the Study:
- To investigate collagen types I and III (COL1, COL3) and IL33 transcript levels in jenny endometrium.
- To histologically localize and quantify COL1 and COL3 proteins.
- To count eosinophils and neutrophils and correlate them with collagen deposition and IL33 expression, comparing findings with mare endometrium.
Main Methods:
- Quantification of COL1A2, COL3A1, and IL33 transcripts using molecular techniques.
- Histological assessment for COL1 and COL3 protein localization and area quantification.
- Manual counting of eosinophils and neutrophils in endometrial tissues.
- Comparative analysis between jenny and mare endometrial collagen distribution.
Main Results:
- Eosinophil and neutrophil counts decreased as fibrosis increased (P < 0.05).
- IL33 transcripts showed a 5-fold increase from categories IIA to III, with a trend towards positive correlation with eosinophils in category IIA.
- COL1 and COL3 protein distribution differed between jennies and mares; COL3 was present in the stratum compactum in jennies, while COL1 was mainly in the deep stroma. Neutrophil counts correlated with COL1 and COL3 areas in jennies, suggesting a role in fibrogenesis.
Conclusions:
- Fibrosis progression in jenny endometrium is associated with decreased eosinophil and neutrophil counts.
- Increased IL33 transcripts, potentially from fibroblasts, may drive fibrosis as eosinophilia subsides.
- Distinct collagen distribution in jenny endometrium warrants further investigation regarding its impact on endometrial function and fertility.
