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A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells

Ce Zhang1, Yue Chen1, Shijin Sun1,2

  • 1Cyrus Tang Hematology Center, Collaborative Innovation Center of Hematology, State Key Laboratory of Radiation Medicine and Prevention, Soochow University, Suzhou, China.

Elife
|November 2, 2020
PubMed

Insights

Researchers discovered a DSSDE motif that directs specific proteins, like corin and CD320, to the apical membrane in kidney epithelial cells via a Rab11a-dependent pathway.

Area of Science:

  • Cell biology
  • Molecular biology
  • Biochemistry

Background:

  • Selective protein distribution is crucial for epithelial cell function.
  • Mechanisms for targeting proteins to specific cell surfaces remain incompletely understood.

Purpose of the Study:

  • To identify the molecular mechanisms regulating apical membrane targeting of proteins containing LDL-receptor (LDLR) modules in renal epithelial cells.
  • To investigate the role of the DSSDE motif in protein localization.

Main Methods:

  • Utilized polarized Madin-Darby canine kidney (MDCK) cells.
  • Investigated the function of the DSSDE motif by altering its sequence.
  • Examined the involvement of Rab11a and Rab11b in protein sorting.

Main Results:

  • A conserved DSSDE motif in LDLR modules of corin and CD320 dictates apical membrane targeting in renal epithelial cells.
  • Altering the DSSDE motif disrupts apical localization of corin and CD320.
  • The DSSDE motif functions in a Rab11a-dependent mechanism for apical sorting.

Conclusions:

  • Revealed a novel molecular recognition mechanism for apical targeting of LDLR module-containing proteins.
  • The DSSDE motif and Rab11a are key regulators of protein localization in polarized epithelial cells.
Keywords:
CD320Rab11aapical expressioncell biologycorinhumanldl receptor module

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