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Wavelength-Tunable Optical Fiber Localized Surface Plasmon Resonance Biosensor via a Diblock Copolymer-Templated
Mengdi Lu1, Hu Zhu2, Long Hong3
1College of Physics, Dalian University of Technology, Dalian 116024, China.
ACS Applied Materials & Interfaces
|November 2, 2020
Summary
Gold nanorods on optical fibers enhance biosensor performance. A self-assembly method improves sensitivity and detection limits for applications like antigen-antibody interactions and DNA sensing.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Materials Science
Background:
- Localized surface plasmon resonance (LSPR) biosensors are crucial for sensitive biomolecule detection.
- Gold nanorods (AuNRs) offer tunable optical properties for LSPR applications.
- Efficient immobilization of AuNRs on sensor platforms is key to enhancing performance.
Purpose of the Study:
- To develop a self-assembly method for well-dispersed and dense AuNR layers on optical fibers.
- To investigate the impact of AuNR aspect ratio and coverage on LSPR biosensor performance.
- To demonstrate the enhanced sensing capabilities and broad applicability of the developed biosensor.
Main Methods:
- Fabrication of AuNR layers on optical fibers using a poly(styrene)-block-poly(acrylic acid) (PS-b-PAA) diblock copolymer.
- Characterization of AuNR properties, including aspect ratio (2.33–4.60) and interparticle gap.
- Optical property analysis and refractive index (RI) sensitivity measurements.
- Performance evaluation using human immunoglobulin G (IgG) detection and other biomolecular assays.
Main Results:
- Achieved well-dispersed and dense AuNR layers, significantly enhancing LSPR biosensor performance.
- Demonstrated that AuNR aspect ratio, deposition time, and interparticle gap critically influence optical properties.
- Attained a maximum RI sensitivity of 753 nm/RIU and a low limit of detection (LOD) of 0.8 nM for human IgG.
- The PS-b-PAA method yielded approximately 3-fold higher RI sensitivity compared to conventional methods due to better particle coverage and reduced aggregation.
Conclusions:
- The self-assembly method provides a robust platform for creating high-performance LSPR biosensors.
- Tuning AuNR aspect ratios allows for regulation of the sensor's detection range.
- The developed AuNR-based LSPR sensors show significant potential for quantitative analysis in antigen-antibody interactions, DNA sensing, and surface-enhanced Raman scattering (SERS).
