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SARS-CoV-2 Detection in Gingival Crevicular Fluid.
S Gupta1, R Mohindra2, P K Chauhan3
1Unit of Periodontics, Oral Health Sciences Centre, Post Graduate Institute of Medical Education and Research (PGIMER), Chandigarh, India.
Journal of Dental Research
|November 3, 2020
Summary
Gingival crevicular fluid (GCF) can detect SARS-CoV-2, similar to saliva, offering insights into COVID-19 oral health connections. This study found GCF is a viable diagnostic fluid for severe acute respiratory syndrome coronavirus 2.
Area of Science:
- Virology
- Oral Medicine
- Infectious Diseases
Background:
- Understanding COVID-19 pathophysiology is crucial.
- Gingival crevicular fluid (GCF) reflects systemic health and can detect viruses.
- SARS-CoV-2 RNA detection in GCF is unexplored.
Purpose of the Study:
- To assess GCF for SARS-CoV-2 presence in COVID-19 patients.
- To compare GCF sensitivity with saliva for SARS-CoV-2 detection.
Main Methods:
- Collected GCF and saliva from 33 COVID-19 positive patients.
- Extracted total RNA and performed RT-qPCR for SARS-CoV-2 E gene.
- Used nasopharyngeal swabs as the gold standard.
Main Results:
- SARS-CoV-2 RNA was detected in GCF of a majority of patients.
- GCF sensitivity was 63.64% and saliva sensitivity was 64.52%.
- GCF and saliva showed comparable sensitivity for SARS-CoV-2 detection.
Conclusions:
- GCF is a potential diagnostic fluid for SARS-CoV-2.
- GCF may represent a possible transmission route for SARS-CoV-2.
- This study provides evidence linking COVID-19 infection and oral health.
