Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Early Versus Late Recurrence in Olfactory Neuroblastoma: A Multi-Institutional Analysis of Predictive Risk Factors.

International forum of allergy & rhinology·2026
Same author

Scoping Review of Global Kidney Genetics Clinic Models and Outcomes.

Kidney international reports·2026
Same author

Exploring fibronodular hepatocellular carcinoma: a case series investigating histopathologic and clinical characteristics and its potential association with cirrhosis.

American journal of clinical pathology·2026
Same author

KCNQ2 neonatal epilepsy: Impact of prompt diagnosis and treatment, and early predictors of outcome severity.

Epilepsia open·2026
Same author

Clinical application of artificial intelligence algorithms in detecting clival remodeling in the setting of pituitary neuroendocrine tumors/pituitary adenomas.

Frontiers in neurology·2026
Same author

Managing Alpha-Gal Allergy in a Case of Bioprosthetic TAVR.

Case reports in immunology·2026

Related Experiment Video

Updated: Dec 2, 2025

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
10:50

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Published on: March 26, 2019

8.1K

[Met5]-enkephalin preserves diffusion metrics in EAE mice.

Chirag Patel1, Mark D Meadowcroft2, Ian S Zagon1

  • 1Department of Neural and Behavioral Sciences, The Pennsylvania State University College of Medicine Hershey, PA, 17033, USA.

Brain Research Bulletin
|November 3, 2020
PubMed
Summary

This study shows that non-invasive imaging, serum [Met5]-enkephalin levels, and behavior can track multiple sclerosis (MS) progression. Treatments like low dose naltrexone (LDN) and Opioid Growth Factor (OGF) improved disease markers in mice.

Keywords:
Diffusion tensor imagingExperimental autoimmune encephalomyelitisLow dose naltrexoneOGFSpinal cord morphology[Met(5)]-enkephalin

More Related Videos

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis
06:19

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis

Published on: September 9, 2022

4.2K
Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis
13:02

Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis

Published on: November 4, 2017

9.0K

Related Experiment Videos

Last Updated: Dec 2, 2025

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo
10:50

Visualizing Impairment of the Endothelial and Glial Barriers of the Neurovascular Unit during Experimental Autoimmune Encephalomyelitis In Vivo

Published on: March 26, 2019

8.1K
Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis
06:19

Induction and Diverse Assessment Indicators of Experimental Autoimmune Encephalomyelitis

Published on: September 9, 2022

4.2K
Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis
13:02

Sagittal Plane Kinematic Gait Analysis in C57BL/6 Mice Subjected to MOG35-55 Induced Experimental Autoimmune Encephalomyelitis

Published on: November 4, 2017

9.0K

Area of Science:

  • Neuroscience
  • Biomarker Discovery
  • Medical Imaging

Background:

  • Multiple sclerosis (MS) lacks specific biomarkers for disease progression and treatment response.
  • Identifying reliable biomarkers is crucial for managing this chronic neurological disorder.

Purpose of the Study:

  • To investigate the correlation between non-invasive imaging, animal behavior, and morphological indicators of disease.
  • To assess the impact of [Met5]-enkephalin (Opioid Growth Factor, OGF) and low dose naltrexone (LDN) on disease progression in an experimental autoimmune encephalomyelitis (EAE) model.

Main Methods:

  • Used the EAE mouse model, treating with OGF, LDN, or saline.
  • Monitored behavior daily, performed non-invasive imaging (MRI), and collected serum and spinal cord tissue.
  • Analyzed serum OGF levels, spinal cord myelin basic protein (MBP), and oligodendrocyte counts (Olig2).

Main Results:

  • Saline-treated EAE mice showed reduced serum OGF and significant demyelination (16% less myelin).
  • OGF and LDN treatments restored MBP and oligodendrocyte levels to normal, indicating reduced demyelination.
  • Non-invasive imaging showed changes in diffusivity metrics, correlating with disease progression and treatment effects.

Conclusions:

  • A panel of non-invasive biomarkers, including imaging, serum OGF, and behavior, correlates with MS disease progression.
  • OGF and LDN treatments demonstrate potential in mitigating demyelination and restoring oligodendrocyte populations.
  • These findings support the validation of specific non-invasive markers for monitoring MS.