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Related Concept Videos

Membrane Domains01:18

Membrane Domains

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The membrane domains concentrate specific lipids and proteins at one place within the membrane, which helps in cell signaling, adhesion, and other critical cellular processes. These domains can differ in size, composition, function, and lifespan.
Protein Domains
The membrane comprises a group of distinct proteins responsible for carrying out a cell's specific function. For example, the plasma membrane of the human sperm, or a single germ cell, contains a unique set of proteins in the...
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Mechanisms of Membrane Domain Formation00:59

Mechanisms of Membrane Domain Formation

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Different physical properties of lipids and proteins allow them to localize and form distinct islands or domains in the membrane. Some membrane domains are formed due to protein-protein interactions, whereas others are formed due to the presence of specific lipids such as sphingolipids and sterols—for example, large proteins, such as bacteriorhodopsin, aggregate and create distinct domains.
Another mechanism for membrane domain formation involves membrane proteins interacting with...
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Fungal Phylum Microsporidia01:28

Fungal Phylum Microsporidia

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Microsporidia are a group of obligate intracellular fungi that were initially classified as protists but were later reclassified based on phylogenetic, molecular, and structural evidence linking them to the Chytridiomycota. These unicellular, non-motile organisms are highly specialized parasites that infect a wide range of animal hosts, including humans. They have evolved extensive genomic and metabolic reductions, making them highly dependent on their hosts for survival.Morphology and Genomic...
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Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

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Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
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Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

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Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
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Membrane Lipids01:32

Membrane Lipids

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Lipids are an essential component of all biological membranes. The average lipid content in mammalian membranes is 50%, though it can be as low as 20% in the inner mitochondrial membrane or as high as 80% in the myelin sheath present around the nerve cells.
Phosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, and sphingomyelin are the most common phospholipids present in mammalian membranes. At physiological pH, phosphatidylserine is negatively charged, while the other three...
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Updated: Dec 2, 2025

Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis
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Lipid Index Determination by Liquid Fluorescence Recovery in the Fungal Pathogen Ustilago Maydis

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Sphingolipid-enriched domains in fungi.

Filipa C Santos1, Joaquim T Marquês1, Andreia Bento-Oliveira1

  • 1Centro de Química Estrutural, Faculdade de Ciências, Universidade de Lisboa, Campo Grande, Portugal.

FEBS Letters
|November 3, 2020
PubMed
Summary

Fungal plasma membranes contain unique sphingolipid-enriched gel domains crucial for cell structure and function. These domains are vital for fungal physiology and may be targets for antifungal therapies.

Keywords:
Neurospora crassaSaccharomyces cerevisiaeantifungal agentsergosterolgel domainsglucosylceramidesinositolphosphorylceramidesmembrane compartments MCC and MCPplasma membranesphingolipid domains

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Area of Science:

  • Cell Biology
  • Biophysics
  • Mycology

Background:

  • Plasma membranes are organized into specialized domains vital for cellular functions.
  • Sphingolipids are key components of plasma membranes, influencing their structure and organization.
  • Fungal plasma membranes exhibit unique sphingolipid-enriched gel domains with distinct biophysical properties.

Purpose of the Study:

  • To review the biophysical features and detection methods of fungal sphingolipid-enriched gel domains.
  • To illustrate the importance of these domains in unicellular and multicellular fungi.
  • To explore the biological roles and potential antifungal implications of these domains.

Main Methods:

  • Analysis of fungal plasma membrane sphingolipidome.
  • Characterization of biophysical properties of gel domains.
  • Review of experimental methods for gel domain detection.

Main Results:

  • Sphingolipid-enriched gel domains are characterized by tightly packed lipids and depleted ergosterol.
  • These domains play roles in fungal stress responses and plasma membrane compartmentalization.
  • A biophysical link between these domains and antifungal compound action is proposed.

Conclusions:

  • Fungal sphingolipid-enriched gel domains are critical for fungal cell integrity and function.
  • Understanding these domains offers insights into fungal physiology and stress adaptation.
  • These domains represent a potential target for developing novel antifungal strategies.