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Updated: Dec 2, 2025

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Bladder cancer-associated transcript 2 contributes to nephroblastoma progression.

Jie Zhu1, Zhenwei Zhu1, Peng Cai1

  • 1Department of Pediatric Surgery, Children's Hospital of Soochow University, Su Zhou, Jiangsu, China.

The Journal of Gene Medicine
|November 3, 2020
PubMed
Summary
This summary is machine-generated.

Long non-coding RNA BLACAT2 promotes nephroblastoma by sponging miR-504-3p, leading to Wnt11 upregulation. This study reveals a novel molecular axis in pediatric kidney cancer development.

Keywords:
gene expressiononcologyrenal/bladder cancer

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Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Nephroblastoma is a common pediatric kidney tumor.
  • Long non-coding RNAs (lncRNAs) are implicated in tumorigenesis.
  • The role of lncRNA BLACAT2 in nephroblastoma remains unclear.

Purpose of the Study:

  • Investigate the role of lncRNA BLACAT2 in nephroblastoma.
  • Determine the association between BLACAT2 expression and patient prognosis.
  • Elucidate the molecular mechanism of BLACAT2 in nephroblastoma.

Main Methods:

  • Analyzed BLACAT2 expression in 50 nephroblastoma tissues and adjacent non-tumorous tissues.
  • Utilized quantitative real-time PCR to detect BLACAT2 levels in cell lines.
  • Employed RNA interference in vitro and in vivo to study BLACAT2 function.
  • Predicted and verified microRNA targets of BLACAT2 and their downstream genes.

Main Results:

  • BLACAT2 silencing inhibited cell proliferation, colony formation, and tumor growth, while promoting apoptosis.
  • BLACAT2 directly bound to miR-504-3p, reducing its expression.
  • miR-504-3p's effects on proliferation and apoptosis were reversed by BLACAT2.
  • Wnt11 was identified as a target gene of miR-504-3p.

Conclusions:

  • A novel BLACAT2/miR-504-3p/Wnt11 axis is involved in nephroblastoma.
  • BLACAT2 acts as a sponge for miR-504-3p, downregulating Wnt11 expression.
  • This axis represents a potential therapeutic target for nephroblastoma.