Transcriptomic alterations induced by aflatoxin B1 and ochratoxin A in LMH cell line
So-Young Choi1, Tae Hyun Kim2, Min-Wook Hong1
1Department of Animal Life Science, Kangwon National University, Chuncheon-si, Gangwon-do 24341, Korea.
Poultry Science
|November 4, 2020
Summary
This study reveals that Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) exhibit antagonistic effects in chicken liver cells, sharing similar toxic mechanisms. Transcriptomic analysis identified key pathways involved in their toxicity.
Area of Science:
- Toxicology
- Molecular Biology
- Poultry Science
Background:
- Aflatoxin B1 (AFB1) and Ochratoxin A (OTA) are toxic mold metabolites with known adverse effects in humans and animals.
- Poultry are sensitive to AFB1 and OTA, but their molecular mechanisms in chickens remain underexplored.
Purpose of the Study:
- To elucidate the molecular mechanisms of AFB1 and/or OTA toxicity in chicken hepatic cells.
- To investigate the potential antagonistic interactions between AFB1 and OTA in poultry.
Main Methods:
- Transcriptomic analysis (mRNA sequencing) of chicken hepatocarcinoma cells exposed to AFB1 and/or OTA.
- Dose-dependent cytotoxicity assays.
- Functional pathway analysis.
Main Results:
- AFB1 and OTA induced cytotoxic effects in a dose-dependent manner.
- Correlation analysis indicated an antagonistic interaction between AFB1 and OTA.
- Commonly regulated pathways included PPAR signaling, focal adhesion, and MAPK signaling.
Conclusions:
- AFB1 and OTA likely share similar toxic mechanisms and compete in chicken livers, leading to antagonistic effects.
- Identified genes provide targets for understanding mycotoxin toxicity and improving poultry health.
