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Related Experiment Video

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Multi-locus Variable-number Tandem-repeat Analysis of the Fish-pathogenic Bacterium Yersinia ruckeri by Multiplex PCR and Capillary Electrophoresis
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Consensus Multilocus Sequence Typing Scheme for Pneumocystis jirovecii.

Lana Pasic1,2,3, Lidia Goterris4,5, Mercedes Guerrero-Murillo4,5

  • 1Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Clinical School, Sydney Medical School, Faculty of Medicine and Health, The University of Sydney, Sydney 2006, Australia.

Journal of Fungi (Basel, Switzerland)
|November 4, 2020
PubMed
Summary
This summary is machine-generated.

A new consensus multilocus sequence typing (MLST) scheme for Pneumocystis jirovecii provides standardized genotyping. This method improves global comparisons and epidemiological studies of this opportunistic fungal pathogen.

Keywords:
CYBP. jiroveciiconsensus MLST schememt26S and SODβ-TUB

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Area of Science:

  • Medical Mycology
  • Infectious Diseases
  • Molecular Epidemiology

Background:

  • Pneumocystis jirovecii causes severe pneumonia in immunocompromised individuals.
  • Multilocus sequence typing (MLST) is crucial for P. jirovecii genotyping but lacks a global standard.
  • Existing MLST schemes hinder large-scale population studies and database development.

Purpose of the Study:

  • To develop a standardized, consensus MLST scheme for Pneumocystis jirovecii.
  • To identify optimal genetic loci for accurate and discriminatory P. jirovecii typing.
  • To facilitate global epidemiological investigations of P. jirovecii infections.

Main Methods:

  • Comparative analysis of 19 genetic loci across 31 published P. jirovecii MLST schemes.
  • Evaluation of eight diverse and commonly used loci for amplification, sequencing, and discriminatory power.
  • Validation of the proposed consensus scheme in identifying related and unrelated P. jirovecii isolates.

Main Results:

  • A new consensus MLST scheme using four loci (β-TUB, CYB, mt26S, SOD) was established.
  • The proposed scheme successfully amplified low and high fungal burden specimens.
  • The scheme demonstrated adequate discriminatory power and accurately identified related/unrelated isolates.

Conclusions:

  • The proposed four-locus consensus MLST scheme offers standardized P. jirovecii typing.
  • This standardized approach will enable robust global epidemiological studies and outbreak investigations.
  • The consensus scheme is a vital tool for understanding the spread of this significant human fungal pathogen.