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Updated: Dec 2, 2025
Chick ex ovo Culture and ex ovo CAM Assay: How it Really Works
Published on: November 30, 2009
The MSC-MCF-7 Duet Playing Tumor Vasculogenesis and Angiogenesis onto the Chick Embryo Chorioallantoic Membrane
Şerban ComŞa1,2, Amalia-Raluca CeauȘu1,2, Roxana Popescu3
1Department of Microscopic Morphology/Histology, "Victor Babeş" University of Medicine and Pharmacy, Timişoara, Romania.
Background/Aim:
Human mesenchymal stem cells (hMSC) represent a versatile cell population, able to modulate the tumor microenvironment. Our aim was to recreate an open scene for the in vivo interaction between hMSC and the MCF-7 breast cancer cells (MCF-7), in order to enlighten the intimate involvement of hMSC in tumor vasculogenesis and angiogenesis.
Materials And Methods:
hMSC and MCF-7 were seeded onto the chick embryo chorioallantoic membrane (CAM) and incubated for 7 days. Consecutively, the morphology and the immunohistochemical profile of CAM were assessed.
Results:
Following this complex interaction, MCF-7 acquired a more aggressive phenotype, hMSC switched to a vascular precursor phenotype, while CAM underwent a major reset to an earlier stage, with hotspots of angiogenesis, vasculogenesis and hematopoiesis.
Conclusion:
The hallmark of this study was the establishment of a veritable in vivo experimental model of MSC involvement in tumor vasculogenesis and angiogenesis, allowing further analysis in the field.
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