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Genetically-encoded Molecular Probes to Study G Protein-coupled Receptors

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Using Ligand-Accelerated Catalysis to Repurpose Fluorogenic Reactions for Platinum or Copper.

Dianne Pham¹, Carly J Deter¹, Mariah C Reinard¹

¹Department of Chemistry, University of Pittsburgh, 219 Parkman Avenue, Pittsburgh, Pennsylvania 15260, United States.

ACS Central Science

|November 4, 2020

Summary

Researchers developed a new catalytic method to repurpose fluorescent probes for detecting metals like platinum and copper. This approach offers enhanced sensitivity and has been applied to various samples, including live cells.

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Preparation of Silver-Palladium Alloyed Nanoparticles for Plasmonic Catalysis under Visible-Light Illumination

Preparation of Silver-Palladium Alloyed Nanoparticles for Plasmonic Catalysis under Visible-Light Illumination

Published on: August 18, 2020

Area of Science:

Chemical Biology
Analytical Chemistry
Materials Science

Background:

Fluorescent probes for metal detection typically rely on chelation or reactivity.
Catalysis-based fluorescent probes offer higher sensitivity, but biocompatible turn-on switches are scarce.

Purpose of the Study:

To develop a novel approach for engineering metal selectivity in fluorescent probes using ligand-accelerated metal catalysis.
To repurpose existing fluorescent probes for detecting different metal ions.

Main Methods:

Exploited ligand-accelerated metal catalysis by repurposing palladium-based probes.
Utilized the cleavage of allylic and propargylic ethers as reaction platforms.
Conducted a combinatorial experiment screening over 800 metal-ligand combinations.

Main Results:

Discovered novel platinum- and copper-selective catalytic methods driven by specific phosphine ligands.
Achieved strong fluorescence signals due to catalytic turnover.
Applied the developed fluorometric technologies to diverse samples, including serum and live cells.

Conclusions:

Ligand-accelerated catalysis provides a new strategy for developing selective fluorescent metal probes.
Demonstrated platinum accumulation in lysosomes of cisplatin-resistant cells, independent of copper.
The findings may guide future engineering of metal selectivity in probe design.