Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Conservative Site-specific Recombination and Phase Variation

Conservative Site-specific Recombination and Phase Variation

Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Co-activators and Co-repressors

Co-activators and Co-repressors

Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...

Eukaryotic Transcription Inhibitors

Eukaryotic Transcription Inhibitors

Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
Eukaryotic transcription inhibitors usually contain two distinct domains, a...

Prokaryotic Transcriptional Activators and Repressors

Prokaryotic Transcriptional Activators and Repressors

The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

[Corticothalamic Circuit Mechanisms Underlying the Regulation of Associative Memory].

Brain and nerve = Shinkei kenkyu no shinpo·2026

Same author

Structural Control of Dual Emission in Coumarin Fluorophores for Visualizing Protein Droplet Maturation.

Angewandte Chemie (International ed. in English)·2026

Same author

Cage-Type Porous Organic Salts as Ship-in-a-Bottle Nanoreactors for Light-Transparent and Reusable Molecular Photocatalysts.

Angewandte Chemie (International ed. in English)·2026

Same author

Development of Sub-50 nm Core-Shell Silica Nanoparticles With Controlled In Vivo Behavior for <sup>19</sup>F Magnetic Resonance Imaging.

Angewandte Chemie (International ed. in English)·2026

Same author

Kupffer Cell Capture-Evading Modifiable Sub-20 nm Lipid Nanodisc-Based <sup>19</sup>F Magnetic Resonance Imaging Probes.

Journal of the American Chemical Society·2026

Same author

Isotonic and minimally invasive optical clearing media for live cell imaging ex vivo and in vivo.

Nature methods·2026

Search research articles

Related Experiment Video

Updated: Dec 2, 2025

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

A Photodeactivatable Antagonist for Controlling CREB-Dependent Gene Expression.

Takuma Imoto¹, Masafumi Minoshima¹, Tatsushi Yokoyama²

¹Division of Advanced Science and Biotechnology, Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.

ACS Central Science

|November 4, 2020

Summary

This summary is machine-generated.

Researchers developed a new light-activated gene control method. This strategy uses light to control the cAMP response element binding protein (CREB) for temporal gene expression regulation.

More Related Videos

Inducible and Reversible Dominant-negative DN Protein Inhibition

Inducible and Reversible Dominant-negative DN Protein Inhibition

Published on: January 7, 2019

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Related Experiment Videos

Last Updated: Dec 2, 2025

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Light-mediated Reversible Modulation of the Mitogen-activated Protein Kinase Pathway during Cell Differentiation and Xenopus Embryonic Development

Published on: June 15, 2017

Inducible and Reversible Dominant-negative DN Protein Inhibition

Inducible and Reversible Dominant-negative DN Protein Inhibition

Published on: January 7, 2019

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

In vivo Application of the REMOTE-control System for the Manipulation of Endogenous Gene Expression

Published on: March 29, 2019

Area of Science:

Molecular Biology
Gene Regulation
Photochemistry

Background:

Controlling gene expression is crucial for biological research and therapeutic applications.
Existing methods for gene control often lack temporal precision.
The cAMP response element binding protein (CREB) pathway is a key regulator of gene transcription.

Purpose of the Study:

To develop a novel photodeactivation strategy for precise temporal control of gene expression.
To investigate the light-induced activation of CREB for gene regulation.
To enable light-triggered recovery of CREB-dependent transcriptional activity.

Main Methods:

Development of a photoresponsive protecting group for a CREB antagonist.
Light-induced cleavage of the protecting group to release the antagonist.
Photodissociation enabling protein-protein interactions between CREB and CBP.
Irradiation of antagonist-treated HEK293T cells to induce gene expression.

Main Results:

Successful light-induced cleavage of the photoresponsive protecting group.
Photocleaved products exhibited weak binding affinity for CBP.
Restoration of protein-protein interactions between CREB and CBP.
Temporal recovery of CREB-dependent transcriptional activity and endogenous gene expression was achieved.

Conclusions:

A novel photodeactivation strategy for controlling gene expression has been established.
Light can be used to temporally regulate CREB-dependent gene transcription.
This method offers precise photolytic control over endogenous gene expression.