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Updated: Dec 2, 2025

Physical, Chemical and Biological Characterization of Six Biochars Produced for the Remediation of Contaminated Sites
Published on: November 28, 2014
Efficient nitrate removal by Pseudomonas mendocina GL6 immobilized on biochar
Wen Zhang1, Jianing Shen1, Huifen Zhang1
1State Key Laboratory of Pollution Control and Resource Reuse, School of the Environment, Nanjing University, Nanjing 210023, PR China.
Abstract:
The performance of nitrate removal by Pseudomonas mendocina GL6 cells immobilized on bamboo biochar was investigated. The results showed that immobilized bacterial cells performed better nitrate removal than the free bacterial cells, and the nitrate removal rate increased from 6.51 mg/(L·h) of free cells to 8.34 mg/(L·h) of immobilized cells. The nitrate removal of immobilized bacterial cells fitted well to the zero-order kinetics model. Moreover, bath experiments showed that immobilized bacterial cells displayed more nitrate removal capacity under different conditions than free bacterial cells due to the protection of biochar carrier. The subsequent mechanistic study suggested that biochar promoted the expression level of denitrification functional genes (napA and nirK) and electron transfer genes involved in denitrification (napB and napC), which resulted in the increase of nitrate removal efficiency. Thus, biochar-immobilized P. mendocina GL6 has much potential to remove nitrate from wastewater via aerobic denitrification.
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