Spectrophotometric Evaluation of Shade Selection with Digital and Visual Methods
Related Experiment Video
Updated: Dec 2, 2025

Evaluation of Photosynthetic Behaviors by Simultaneous Measurements of Leaf Reflectance and Chlorophyll Fluorescence Analyses
Published on: August 9, 2019
S Preethi Suganya1, P Manimaran1, D Saisadan1
1Department of Prosthodontics, JKKN Dental College & Hospital, Komarapalayam, Tamil Nadu, India.
Aim:
The main purpose of the study was to learn the possible shade differences between human eye and the digital method with spectrophotometric methods.
Materials And Methods:
Fifty dental students of age groups 18-25 with maxillary right central incisor after undergoing oral prophylaxis were included in this study. Before undergoing shade selection, standardization protocol was followed. The shade of the right maxillary central incisor was determined by using the following methods: visual, spectrophotometer, and digital photography. The shades obtained by both spectrophotometer and visual methods are compared and similarly L*, a*, and b* values obtained by both spectrophotometer and digital methods are compared. Finally these comparisons are subjected to statistical analysis.
Results:
Z and P values of digital and spectrophotometric methods were found to be statistically significant. Tooth shade selected between spectrophotometric and visual method was analyzed by using chi-square test, which shows highly significant value.
Conclusion:
It was concluded that the digital photography can be used as one of the reliable methods for shade selection in a clinical setup.
More Related Videos
Related Concept Videos
UV–Vis Spectrometers
Spectrophotometry: Introduction
The essential components of a spectrophotometer include a source of electromagnetic radiation, a slot for placing a material to be analyzed, and a...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview
Photoreceptors and Plant Responses to Light
UV–Vis Spectroscopy of Conjugated Systems
One of the factors influencing λmax is the extent of conjugation in...

