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Novel Fast and Reliable Method for Nano-Erythrosome Production Using Shear Force.

Simona Capossela1, Vikas Mathew1, Manuela Boos2

  • 1SCI Biobanking and Translational Medicine, Swiss Paraplegic Research, Nottwil, Switzerland.

Drug Design, Development and Therapy
|November 5, 2020
PubMed
Summary
This summary is machine-generated.

A novel shear force method offers a faster, easier, and more reproducible way to produce nano-erythrosomes (NEs) compared to traditional extrusion. This new technique ensures sterility and produces stable NEs, paving the way for large-scale production.

Keywords:
erythrocytesextrusionnanoerythrosomesnanotechnologynew methodshear force

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Area of Science:

  • Biomaterials Science
  • Nanotechnology
  • Drug Delivery Systems

Background:

  • Conventional nano-erythrosome (NE) production via extrusion faces challenges including complex equipment, lengthy procedures, inconsistent pressure, and sterility concerns.
  • Alternative methods like ultrasound probe sonication have shown limitations such as sample overheating and suboptimal NE characteristics compared to extrusion.

Purpose of the Study:

  • To propose, develop, and evaluate a novel shear force-based method for nano-erythrosome (NE) fabrication.
  • To compare the efficacy and characteristics of NEs produced by the new shear force method against the established extrusion technique.

Main Methods:

  • Employed mechanical shear force using a rotor-stator tissue homogenizer to disrupt hemoglobin-depleted erythrocyte ghost membranes.
  • Utilized identical batches of erythrocyte ghost membranes to directly compare shear force-produced NEs with extrusion-produced NEs.
  • Characterized NEs for yield, size, encapsulation efficiency, morphology, and stability using flow cytometry, transmission electron microscopy, and zeta potential analysis.

Main Results:

  • The shear force method demonstrated enhanced ease of setup, significantly reduced procedure time, improved sterility control, and decreased batch-to-batch variability.
  • Shear force-generated NEs exhibited a desirable size distribution (~125 nm) and were morphologically and functionally equivalent to extrusion-derived NEs.
  • NEs produced by shear force displayed excellent stability (counts, size, fluorescence) for 3 weeks at +4°C, along with good colloidal stability and resistance to stress.

Conclusions:

  • The novel shear force method provides a faster, simpler, and highly reproducible approach for NE production compared to extrusion.
  • This method facilitates simultaneous sterile batch production of NEs with homogenous size, enhanced stability, and improved shelf-life.
  • The shear force technique's capacity for high-concentration sample processing suggests significant potential for future large-scale NE production and industrial applications.