Microarray Expression Profiles and Bioinformatics Analyses Reveal Aberrant Circular RNAs Expression in Bladder Cancer
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Updated: Dec 2, 2025
Identification of Circular RNAs using RNA Sequencing
Published on: November 14, 2019
Jun Yang1, Junwen Chen2, Si Wu3
1Department of Gastroenterology, Shengjing Hospital of China Medical University, Shenyang 110004, People's Republic of China.
Background:
Increasing evidence shows that circular RNAs (circRNAs) are involved in many biological processes, functioning as microRNA (miRNA) sponges. The aim of this study is to identify differentially expressed circRNAs in bladder cancer (BCa).
Methods:
The transcriptome of circRNAs in BCa was assayed by microarray. Quantitative real-time PCR was performed to verify the results. Then, potential miRNA response elements (MREs) between circRNAs and miRNAs were predicted. Pathway and ontology enrichment analyses were performed to identify mechanisms related to the gene regulation of differentially expressed circRNAs.
Results:
Three hundred and eighty-six up-regulated and 394 down-regulated circRNAs were identified, and their potential MREs were predicted in BCa.
Conclusion:
The differentially expressed circRNAs indicate that circRNAs could play important roles in the molecular pathogenesis of BCa.
