Automated, cassette-based isolation and formulation of high-purity [⁶¹Cu]CuCl₂ from solid Ni targets

A need for improved, cassette-based automation of ⁶¹Cu separation from irradiated Ni targets was identified given the growing interest in theranostics, and generally lengthy separation chemistries for ⁶⁴Cu/⁶⁴Ni, upon which ⁶¹Cu chemistry is often based.

A method for separating ⁶¹Cu from irradiated ^natNi targets was therefore developed, with provision for target recycling. Following deuteron irradiation, electroplated ^natNi targets were remotely transferred from the cyclotron and dissolved in acid. The dissolved target solution was then transferred to an automated FASTlab chemistry module, where sequential TBP and TK201 (Triskem) resins isolated the [⁶¹Cu]CuCl₂, removed Ni, Co, and Fe, and concentrated the product into a formulation suitable for anticipated radiolabelling reactions.

⁶¹Cu saturation yields of 190 ± 33 MBq/μA from energetically thick ^natNi targets were measured. The average, decay-corrected, activity-based dissolution efficiency was 97.5 ± 1.4% with an average radiochemical yield of 90.4 ± 3.2% (N = 5). The isolated activity was collected approximately 65 min post end of bombardment in ~ 2 mL of 0.06 M HCl (HCl concentration was verified by titration). Quality control of the isolated [⁶¹Cu]CuCl₂ (N = 5) measured ⁵⁸Co content of (8.3 ± 0.6) × 10^- 5% vs. ⁶¹Cu by activity, Ni separation factors ≥ (2.2 ± 1.8) × 10⁶, EoB molar activities 85 ± 23 GBq/μmol and NOTA-based EoB apparent molar activities of 31 ± 8 MBq/nmol and 201 MBq/nmol for the 30 min and 3.3 h (N = 1) irradiations, respectively.

High purity ⁶¹Cu was produced with the developed automated method using a single-use, cassette-based approach. It was also applicable for ⁶⁴Cu, as demonstrated with a single proof-of-concept ⁶⁴Ni target production run.