Assessing a co-culture fungal granule ability to remove pharmaceuticals in a sequencing batch reactor

Biodegradation of carbamazepine (CBZ), diclofenac sodium (DCF) and ibuprofen (IBP) was evaluated through fungal granules development in a sequencing batch reactor (SBR). Fungal granules were developed in co-culture of T. polyzona, A. niger, T. longibrachiatum, M. circinelloides and R. microsporus at a retention time (RT) of 2 days and 1 day. Ligninolytic enzymes [laccase (Lac), lignin peroxidase (LiP) and manganese peroxidase (MnP)] were determined. Removal of pharmaceuticals was assessed and metabolites identified using the SPE-UPLC-QToF/MS methods. A pH range of 3-4.6 was found to improve the granulation development from day 6 and the production of ligninolytic enzymes [MnP (253.00 ± 14.19 U/L), Lac (111.58 ± 10.00 U/L) and LiP (95.25 ± 8.22 U/L)]. At steady-state, a removal of 97.41±0.25%, 99.83±0.14%, and 99.91±0.08 were achieved at an RT of 2 days for CBZ, DCF, and IBP, respectively, and of 91.94±0.05%, 99.31±0.12% and 97.72±0.23% at an RT of 1 days for the same PhCs. A variety of chemical reactions have been proposed for degradation pathways catalysed by enzyme-producing fungi, generating fragment ions of intermediate compounds. This study is highly relevant for cost-effective and environmentally friendly wastewater treatment processes in water scare countries.