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A 2D-1H-n.m.r. study of some Shigella flexneri O-polysaccharides
P E Jansson1, L Kenne, T Wehler
1Department of Organic Chemistry, Arrhenius Laboratory, University of Stockholm, Sweden.
Carbohydrate Research
|September 1, 1987
Abstract:
A 1H-n.m.r. study of the O-polysaccharides from different types of Shigella flexneri has been performed. With the aid of 2D-n.m.r. techniques, namely, J-resolved, spin-spin correlation, and NOESY experiments, most of the structural features of these polysaccharides could be deduced. Sequences could generally be obtained from the NOESY experiments. When using a prolonged mixing time in these experiments, cross-peaks due to spin diffusion from one anomeric proton to the anomeric proton of an adjacent residue could be obtained, thereby giving complementary sequence information.