Related Experiment Video
Updated: Dec 2, 2025
07:20
Inducement and Evaluation of a Murine Model of Experimental Myopia
Published on: January 22, 2019
10.3K
PPARγ modulates refractive development and form deprivation myopia in Guinea pigs
Miaozhen Pan1, Zhenqi Guan1, Peter S Reinach1
1School of Ophthalmology and Optometry and Eye Hospital, Wenzhou Medical University, Wenzhou, Zhejiang, China; State Key Laboratory of Optometry, Ophthalmology, and Vision Science, Wenzhou, Zhejiang, China.
Experimental Eye Research
|November 5, 2020
Summary
Peroxisome proliferator-activated receptor γ (PPARγ) influences form deprivation myopia (FDM) by altering choroidal thickness, blood perfusion, and scleral hypoxia. PPARγ modulation impacts collagen expression, offering insights into myopia development.
Area of Science:
- Ophthalmology
- Molecular Biology
- Physiology
Background:
- Form deprivation myopia (FDM) involves reduced choroidal thickness (ChT) and blood perfusion (ChBP), leading to scleral hypoxia.
- Peroxisome proliferator-activated receptor γ (PPARγ) expression levels can modulate hypoxia-induced pathological responses in various tissues.
Purpose of the Study:
- To investigate if PPARγ modulates FDM via changes in ChT, ChBP, and scleral hypoxia-inducible factor-1α (HIF-1α).
- To determine the effect of PPARγ on scleral collagen type 1 (COL1) expression in the context of FDM.
Main Methods:
- Myopia was induced in guinea pigs by ocular occlusion.
- Animals received peribulbar injections of a PPARγ antagonist (GW9662) or agonist (GW1929).
- Ocular refraction, biometric parameters, ChT, ChBP, scleral HIF-1α, and COL1 expression were assessed.
Main Results:
- GW9662 induced myopia and reduced ChT and ChBP, increasing HIF-1α and decreasing COL1 expression.
- GW1929 inhibited FDM progression, attenuated declines in ChT and ChBP, reduced HIF-1α upregulation, and lessened COL1 decrease in occluded eyes.
- PPARγ modulation demonstrated an inverse relationship with HIF-1α expression levels.
Conclusions:
- PPARγ plays a significant role in regulating FDM progression.
- PPARγ influences scleral collagen expression through an inverse relationship with HIF-1α.
- Targeting PPARγ may offer therapeutic strategies for myopia control.
