Lipoxin A4 promotes reduction and antibiotic efficacy against Pseudomonas aeruginosa biofilm

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J M Thornton1, J M Walker1, P Y Kadiyam Sundarasivarao1

  • 1Department of Cell Biology and Neuroscience, Rowan University School of Osteopathic Medicine, Stratford, NJ, USA.

Prostaglandins & Other Lipid Mediators
|November 5, 2020
PubMed

Insights

Specialized Pro-resolving Mediators, Lipoxin A4 (LxA4) and RvD2, inhibit Pseudomonas aeruginosa biofilm formation and virulence. LxA4 also enhances antibiotic efficacy against established biofilms.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Pharmacology

Background:

  • Pseudomonas aeruginosa (P. aeruginosa) forms biofilms, reducing antibiotic efficacy in infections.
  • Lipoxin A4 (LxA4), a Specialized Pro-resolving Mediator (SPM), previously shown to inhibit P. aeruginosa virulence.
  • Biofilm formation is a key factor in P. aeruginosa pathogenesis and treatment resistance.

Purpose of the Study:

  • To investigate the direct effects of LxA4 and RvD2 on P. aeruginosa biofilm formation and virulence.
  • To assess the impact of LxA4 on the efficacy of antibiotics against P. aeruginosa.
  • To evaluate combined effects of LxA4 and antibiotics on biofilm.

Main Methods:

  • Treatment of P. aeruginosa with LxA4 and RvD2.
  • Measurement of biofilm formation and virulence gene expression.
  • Assessment of antibiotic efficacy (ciprofloxacin, imipenem) alone and in combination with LxA4.

Main Results:

  • LxA4 and RvD2 significantly reduced P. aeruginosa biofilm formation and virulence gene expression.
  • LxA4 enhanced ciprofloxacin's inhibition of biofilm formation and imipenem's killing action.
  • LxA4 improved ciprofloxacin's bacterial killing in established P. aeruginosa biofilms.

Conclusions:

  • LxA4 and RvD2 directly inhibit P. aeruginosa biofilm formation and virulence.
  • LxA4 enhances the efficacy of certain antibiotics against P. aeruginosa, including in established biofilms.
Keywords:
AntibioticsLipoxin A4Pseudomonas aeruginosaResolvin D2biofilm

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