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Related Experiment Video

Updated: Dec 2, 2025

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

High-Throughput Metabolic Profiling for Model Refinements of Microalgae

Published on: December 4, 2021

High-Throughput Functional Genomics for Energy Production.

Jacob A Fenster¹, Carrie A Eckert²

¹Chemical and Biological Engineering, University of Colorado, Boulder CO, United States; Renewable and Sustainable Energy Institute, University of Colorado, Boulder CO, United States.

Current Opinion in Biotechnology

|November 5, 2020

Summary

This summary is machine-generated.

High-throughput functional genomics uses trackable mutagenesis to rapidly profile mutant libraries, accelerating synthetic biology for strain engineering. This enables discovering beneficial mutations for developing industrial microbes and a bio-based economy.

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Area of Science:

Synthetic biology
Genomics
Metabolic engineering

Background:

Functional genomics is crucial for understanding genotype-phenotype relationships.
High-throughput (HTP) methods accelerate the Design-Build-Test-Learn cycle in synthetic biology.
Strain engineering relies on identifying beneficial mutations for desired traits.

Purpose of the Study:

To highlight the role of HTP functional genomics in advancing strain engineering.
To demonstrate how trackable mutagenesis accelerates the discovery of beneficial mutations.
To emphasize the potential of these methods for developing industrial microbes.

Main Methods:

Utilizing trackable mutagenesis techniques like transposon insertion sequencing.
Employing CRISPR-Cas-mediated genome editing for rapid fitness profiling.
Implementing iterative rounds of mutagenesis for complex phenotype development.

Main Results:

Accelerated fitness profiling of mutant libraries.
Discovery of beneficial mutations for strain improvement.
Enabling the engineering of complex production and tolerance phenotypes.

Conclusions:

HTP functional genomics and trackable mutagenesis are key drivers of forward synthetic biology.
These methods facilitate the development of next-generation industrial microbes.
Expansion to novel bacteria supports the establishment of a bio-based economy.