Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Corneal rim cultures.

W D Mathers1, M A Lemp

  • 1Cornea Service, Georgetown University, Washington, D.C.

Cornea
|January 1, 1987
PubMed
Summary
This summary is machine-generated.

Scleral rim cultures revealed Staphylococcus epidermidis and Streptococcus viridans resistance to gentamycin. This suggests eyebanks should add a second antibiotic to their media to prevent infections after corneal transplantation.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation and differential diagnosis of keratoconjunctivitis sicca.

The Journal of rheumatology. Supplement·2000
Same author

Why the eye becomes dry: a cornea and lacrimal gland feedback model.

The CLAO journal : official publication of the Contact Lens Association of Ophthalmologists, Inc·2000
Same author

Confirmation of confocal microscopy diagnosis of Acanthamoeba keratitis using polymerase chain reaction analysis.

Archives of ophthalmology (Chicago, Ill. : 1960)·2000
Same author

Exclusion of AR-CHED from the chromosome 20 region containing the PPMD and AD-CHED loci.

Ophthalmic genetics·2000
Same author

The 1998 Castroviejo Lecture. New strategies in the treatment of dry-eye states.

Cornea·1999
Same author

Cytologic changes in the conjunctiva mimicking malignancy after topical mitomycin C chemotherapy.

Ophthalmology·1999
Same journal

Corneal Tissue Addition Keratoplasty.

Cornea·2026
Same journal

Corneal Tissue Addition Keratoplasty for Keratoconus: Point-Counterpoint.

Cornea·2026
Same journal

Posterior Segment Pressurization With Intravitreal Balanced Salt Solution to Stabilize Anterior Chamber Gas During Endothelial Keratoplasty.

Cornea·2026
Same journal

Central Corneal Thickness Six Postoperative Months After DMEK or Triple-DMEK.

Cornea·2026
Same journal

Rebubbling and Graft Detachment in Pre-Descemet Endothelial Keratoplasty: A Prospective Study.

Cornea·2026
Same journal

Letter Regarding: ESCALÓN: A Prospective Randomized Trial of Corneal Endothelial Cell Therapy in Subjects With Corneal Edema.

Cornea·2026
See all related articles

Area of Science:

  • Ophthalmology
  • Microbiology

Background:

  • Corneal transplantation is a common procedure.
  • Eyebank media are crucial for preventing microbial contamination.
  • Antibiotic resistance is a growing concern in healthcare.

Purpose of the Study:

  • To analyze the prevalence and antibiotic resistance patterns of microbial contaminants in scleral rim cultures.
  • To evaluate the efficacy of current eyebank media in preventing bacterial growth.

Main Methods:

  • Retrospective analysis of 291 scleral rim cultures from corneal transplantations performed between 1981 and 1986.
  • Identification of microbial pathogens and assessment of their antibiotic susceptibility, particularly to gentamycin.

Main Results:

Related Experiment Videos

  • 39% of cultures were positive for microbial growth.
  • Staphylococcus epidermidis was the most common pathogen (30%), with 42% resistant to gentamycin.
  • Streptococcus viridans was found in 15 cultures, with 87% resistant to gentamycin but sensitive to other antibiotics.
  • No cases of postoperative endophthalmitis were reported.

    • Conclusions:

    • The study highlights the emergence of gentamycin-resistant strains of Staphylococcus epidermidis and Streptococcus viridans.
    • Current eyebank media may require modification to address rising antibiotic resistance.
    • Consideration should be given to adding a second antibiotic to the MK medium used by eyebanks to enhance graft safety.