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MiR-127-3p inhibits proliferation of ovarian cancer in rats through down-regulating MAPK4

S-Y Du1, X-X Huang, N-M Li

  • 1Department of Obstetrics and Gynecology, Jinan City People's Hospital, Jinan, China. 851256@qq.com.

European Review for Medical and Pharmacological Sciences
|November 6, 2020
PubMed
Abstract

Insights

Micro ribonucleic acid (miR)-127-3p inhibits epithelial ovarian cancer (EOC) by downregulating MAPK4. This discovery offers new therapeutic targets for EOC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gene Regulation

Background:

  • Epithelial ovarian cancer (EOC) remains a leading cause of cancer-related mortality in women.
  • Identifying novel molecular targets for EOC therapy is crucial for improving patient outcomes.
  • MicroRNAs (miRNAs) have emerged as critical regulators of cancer development and progression.

Purpose of the Study:

  • To investigate the anti-tumor role of micro ribonucleic acid (miR)-127-3p in epithelial ovarian cancer (EOC).
  • To elucidate the molecular mechanism underlying the action of miR-127-3p in EOC.
  • To explore the potential of miR-127-3p as a therapeutic agent for EOC.

Main Methods:

  • Quantitative Reverse Transcription-Polymerase Chain Reaction (qRT-PCR) to detect miR-127-3p expression in EOC cell lines and tissues.
  • Lentiviral transfection to overexpress miR-127-3p in EOC cells (OVCAR-3 and Caov-3).
  • In vitro assays including proliferation, bufalin sensitivity, wound healing, and invasion assays to assess anti-tumor effects.
  • Dual-Luciferase reporter assay and qRT-PCR to validate MAPK4 as a direct target of miR-127-3p.
  • Functional studies to explore the role of MAPK4 in EOC cell phenotypes.

Main Results:

  • MiR-127-3p was significantly downregulated in EOC cell lines and clinical samples.
  • Overexpression of miR-127-3p inhibited EOC cell proliferation and invasion, and enhanced sensitivity to bufalin.
  • MiR-127-3p directly targets and regulates the expression of the mitogen-activated protein kinase 4 (MAPK4) gene in EOC.
  • Upregulation of MAPK4 counteracted the anti-tumor effects of miR-127-3p, promoting proliferation and migration while reducing bufalin sensitivity.

Conclusions:

  • MiR-127-3p functions as a tumor suppressor in EOC.
  • The anti-tumor activity of miR-127-3p is mediated through the direct regulation of MAPK4 expression.
  • Targeting the miR-127-3p/MAPK4 axis presents a promising therapeutic strategy for epithelial ovarian cancer.

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