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Circular RNA circ-PRKCI promotes lung cancer progression by binding to microRNA-1324 to regulate MECP2 expression
1Department of Respiratory Medicine, Shandong Shanxian Central Hospital, Heze, China. 12124806@cumt.edu.cn.
European Review for Medical and Pharmacological Sciences
|November 6, 2020
Summary
Circular RNA PRKCI (circ-PRKCI) is highly expressed in lung cancer (LCa), promoting tumor progression and poor prognosis. Inhibition of circ-PRKCI suppresses LCa cell invasiveness and proliferation.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Lung cancer (LCa) remains a leading cause of cancer mortality worldwide.
- Circular RNAs (circRNAs) are increasingly recognized for their roles in cancer development.
- The specific function of circ-PRKCI in LCa pathogenesis is not well understood.
Purpose of the Study:
- To investigate the expression levels of circ-PRKCI in lung cancer.
- To elucidate the molecular mechanism by which circ-PRKCI influences LCa progression.
- To determine the prognostic value of circ-PRKCI in LCa patients.
Main Methods:
- Quantitative Real-Time Polymerase Chain Reaction (qRT-PCR) and Western blotting were used to assess circ-PRKCI and MECP2 expression.
- Cell Counting Kit-8 (CCK-8), clone formation, and transwell invasion assays evaluated cell proliferation and invasiveness.
- Dual-luciferase reporter assays verified the interaction between circ-PRKCI, microRNA-1324, and MECP2.
Main Results:
- Circ-PRKCI expression was significantly elevated in LCa tissues and cell lines compared to normal controls.
- High circ-PRKCI levels correlated with poorer prognosis in LCa patients.
- Inhibition of circ-PRKCI suppressed LCa cell proliferation and invasion in vitro.
- Circ-PRKCI was found to bind to microRNA-1324, competing with MECP2.
Conclusions:
- Circ-PRKCI is upregulated in lung cancer and associated with adverse patient outcomes.
- Circ-PRKCI promotes LCa progression by sponging microRNA-1324 and affecting the MECP2 pathway.
- Circ-PRKCI represents a potential diagnostic biomarker and therapeutic target for lung cancer.
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