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Published on: August 7, 2017
Serum metabolomic profiling reveals important difference between infants with and without subsequent recurrent
Xiaoyan Zhang1,2, Dan Peng2, Xiang Zhang1
1Department of Respiratory Medicine, Beijing Children's Hospital, Capital Medical University, National Center for Children's Health, Beijing, China.
Insights
Serum metabolomics can identify infants with severe respiratory syncytial virus (RSV) bronchiolitis who may develop recurrent wheezing. Elevated L-lactic acid levels are linked to this condition, suggesting potential biomarkers.
Area of Science:
- Biochemistry
- Immunology
- Pediatrics
Background:
- Severe respiratory syncytial virus (RSV) bronchiolitis in infancy is a risk factor for developing recurrent wheezing.
- Understanding the early biological markers of recurrent wheezing is crucial for early intervention.
Purpose of the Study:
- To differentiate infants with severe RSV bronchiolitis who develop subsequent recurrent wheezing using serum metabolomics.
- To explore the association between serum metabolic profiles, host immune responses, and the development of recurrent wheezing.
Main Methods:
- Serum metabolomics analysis using ultra-performance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-MS/MS).
- Measurement of cytokines via multiplex immunoassay in 51 infants hospitalized with severe RSV bronchiolitis.
- Follow-up of infants until 3 years of age to identify development of recurrent wheezing.
Main Results:
- Distinct serum metabolomic profiles were observed between infants who developed recurrent wheezing and those who did not.
- Significantly higher levels of L-lactic acid were found in infants who developed recurrent wheezing.
- Altered pathways included pyrimidine metabolism, glycerophospholipid metabolism, and arginine biosynthesis; L-lactic acid correlated positively with CXCL8 levels.
Conclusions:
- Serum metabolic signatures during severe RSV bronchiolitis in early infancy are associated with later development of recurrent wheezing.
- L-lactic acid may serve as a potential biomarker for predicting recurrent wheezing in infants post-RSV bronchiolitis.
Abstract:
We aimed to use serum metabolomics to discriminate infants with severe respiratory syncytial virus (RSV) bronchiolitis who later developed subsequent recurrent wheezing from those who did not and to investigate the relationship between serum metabolome and host immune responses with regard to the subsequent development of recurrent wheezing. Fifty-one infants who were hospitalized during an initial episode of severe RSV bronchiolitis at 6 months of age or less were included and followed for up to the age of 3 years. Of them, 24 developed subsequent recurrent wheezing and 27 did not. Untargeted serum metabolomics was performed by ultraperformance liquid chromatography coupled with high-resolution mass spectrometry (UPLC-MS/MS). Cytokines were measured by multiplex immunoassay. Difference in serum metabolomic profiles was observed between infants who developed recurrent wheezing and those who did not. L-lactic acid level was significantly higher in infants with recurrent wheezing than those without. Pyrimidine metabolism, glycerophospholipid metabolism, and arginine biosynthesis were identified as the most significant changed pathways between the two groups. Moreover, L-lactic acid level was positively associated with serum CXCL8 level. This exploratory study showed that differential serum metabolic signatures during severe RSV bronchiolitis in early infancy were associated with the development of subsequent recurrent wheezing in later childhood.
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