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Functionalization of hybrid surface microparticles for in vitro cellular antigen classification
Brandon K Ashley1, Jianye Sui2, Mehdi Javanmard1,2
1Department of Biomedical Engineering, Rutgers, The State University of New Jersey, Piscataway, NJ, 08854, USA.
Analytical and Bioanalytical Chemistry
|November 6, 2020
Summary
Researchers developed a new method for functionalizing hybrid microparticles with antibodies for biomedical assays. This technique ensures uniform antibody attachment, enabling sensitive detection of disease markers using minimal samples.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Nanotechnology
Background:
- Hybrid material surfaces on microparticles are crucial for multiplexing applications.
- Functionalizing these surfaces with biomolecules presents challenges in uniformity, repeatability, and sample conservation.
- Microenvironmental differences at hybrid interfaces impact functionalization efficiency.
Purpose of the Study:
- To demonstrate and validate streptavidin adsorption-based antibody functionalization on unmodified hybrid surface microparticles for in vitro analysis.
- To optimize surface chemistry for uniform and repeatable biomolecule conjugation.
- To introduce a cost-effective and low-impact method for multiplexing applications.
Main Methods:
- Fabrication of hybrid surface microparticles with a polystyrene core and aluminum oxide semi-coating.
- Optimization of streptavidin-biotin functionalization using analytical mass balances.
- Conjugation of anti-human CD11b antibodies to the functionalized microparticles.
- Characterization using ultraviolet protein absorbance and fluorescence microscopy with multi-sectional imaging (MSI).
Main Results:
- Successful demonstration of antibody functionalization on hybrid surface microparticles.
- Optimization of streptavidin-biotin chemistry for improved assay performance and sample sparing.
- Confirmation of functionalization uniformity using MSI.
- Proof-of-concept validation by detecting human neutrophils from patient blood samples.
Conclusions:
- This study presents a novel functionalization strategy for hybrid surface microparticles.
- The developed method is suitable for in vitro cellular or proteomic multiplexing applications.
- The approach offers advantages of minuscule sample volumes, low cost, and reduced environmental impact.
