Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structure-mutagenicity relationships of benzidine analogues.

E A Messerly1, J E Fekete, D R Wade

  • 1College of Pharmacy, University of Michigan, Ann Arbor 48109-1065.

Environmental and Molecular Mutagenesis
|January 1, 1987
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Adrenal proteins bound by a reactive intermediate of mitotane.

Cancer chemotherapy and pharmacology·1997
Same author

Gas chromatographic-electron capture determination of 2,4'-dichlorodiphenylacetic acid from in-vitro adrenal transformations of mitotane and its analogs.

Journal of pharmaceutical and biomedical analysis·1996
Same author

Induction of benign prostatic hyperplasia in intact dogs by near-physiological levels of 5 alpha-dihydrotestosterone and 17 beta-estradiol.

The Prostate·1995
Same author

Bovine adrenal cortex transformations of mitotane [1-(2-chlorophenyl)-1-(4-chlorophenyl)-2,2-dichloroethane; o,p'-DDD] and its p,p'- and m,p'-isomers.

Biochemical pharmacology·1995
Same author

Metabolic activation and binding of mitotane in adrenal cortex homogenates.

Journal of pharmaceutical sciences·1995
Same author

Substituent effects on the genotoxicity of 4-nitrostilbene derivatives.

Mutation research·1994
Same journal

Evaluating the Suitability of HepaRG Cells for Regulatory Micronucleus Testing: Comparing Results for a Diverse Set of 28 Chemicals to Regulatory Accepted TK6 Cells.

Environmental and molecular mutagenesis·2026
Same journal

Resveratrol Mitigates Haloacetaldehyde Disinfection By-Products (HAL-DBPs)-Induced Oocyte Developmental Toxicity via Mitochondria-Dependent Pathways.

Environmental and molecular mutagenesis·2026
Same journal

DZNep: A Methyltransferase Modulator for Disease Mitigation.

Environmental and molecular mutagenesis·2026
Same journal

Transcriptional Dysregulation of Histone Methyltransferase Genes and Chromosomal Instability Associated With Chronic Pesticide Exposure.

Environmental and molecular mutagenesis·2026
Same journal

Evaluation of Type 1 Error Rates in Duplex Sequencing for Mutagenicity Testing Using Vehicle Control Data and Simulation Analyses.

Environmental and molecular mutagenesis·2026
Same journal

Benzo[b]fluoranthene Induces Mutation Accumulation and Cancer-Relevant Mutational Signatures in Mouse Lung Alongside Steady State Levels of Chromosome Damage in Blood.

Environmental and molecular mutagenesis·2026
See all related articles

Benzidine analogues were tested for mutagenicity using the Ames test. Mutagenic activity, particularly with rat liver S9 activation, varied significantly among compounds, with dichlorobenzidine and 4-aminobiphenyl showing high potency.

Area of Science:

  • Toxicology
  • Genetics
  • Chemical Mutagenesis

Background:

  • Benzidine and its analogues are known environmental contaminants.
  • Assessing their mutagenic potential is crucial for understanding their health risks.

Purpose of the Study:

  • To compare the mutagenic activities of benzidine, its dihydrochloride salt, and twelve analogues.
  • To evaluate the influence of structural modifications and metabolic activation on mutagenicity.

Main Methods:

  • The Ames test was employed using bacterial strains TA100 and TA98.
  • Tests were conducted with and without rat liver S9 fraction to assess metabolic activation.

Main Results:

  • Most compounds showed little to no mutagenicity without S9 activation.

Related Experiment Videos

  • With S9 activation, all compounds except tetramethylbenzidine were active in TA100; dichlorobenzidine and 4-aminobiphenyl were most potent.
  • Bridged diphenyl compounds were less mutagenic in TA98 than planar biphenyls, except for a nitroaniline derivative which was mutagenic in both strains, with or without S9.

    • Conclusions:

    • Mutagenicity of benzidine analogues is structure-dependent and often requires metabolic activation.
    • A decrease in the basicity of parent anilines correlated with increased mutagenicity for certain 3,3'-disubstituted benzidines.