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Large-Amplitude Conformational Changes in Self-Assembled Multi-Stranded Aromatic Sheets.

Joan Atcher1,2, Pedro Mateus2, Brice Kauffmann3

  • 1Department of Pharmacy and Center for Integrated Protein Science, Ludwig-Maximilians-Universität, Butenandtstrasse 5-13, 81377, München, Germany.

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This summary is machine-generated.

Complex synthetic foldamers enable large conformational changes. Researchers designed aromatic sheets that self-assemble into stacked dimers, confirmed by mass spectrometry, X-ray crystallography, and simulations.

Keywords:
aromatic stackingconformational changefoldamersself-assemblysheets

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Area of Science:

  • Synthetic chemistry
  • Supramolecular chemistry
  • Structural biology

Background:

  • Foldamers are synthetic oligomers mimicking proteins.
  • Complex foldamers allow for large conformational changes.
  • Aromatic sheets are a rare but promising foldamer motif.

Purpose of the Study:

  • To design and characterize novel synthetic foldamers with aromatic sheet structures.
  • To investigate the self-assembly and conformational behavior of these foldamers.
  • To explore the potential of aromatic sheets in creating complex supramolecular architectures.

Main Methods:

  • De novo design of aromatic sheet foldamers.
  • Self-assembly studies.
  • Ion-mobility Electrospray Ionization Mass Spectrometry (ESI-MS) for dimer confirmation.
  • X-ray crystallography for structural analysis.
  • Molecular dynamics simulations for conformational stability and interconversion.

Main Results:

  • Successfully designed foldamers self-assemble into discrete stacks of intercalated aromatic strands.
  • Formation of compact dimers was confirmed by ion-mobility ESI-MS.
  • X-ray crystallography identified two distinct dimeric conformational states.
  • Large conformational changes are required for interconversion between these states.
  • Molecular dynamics simulations validated the stability of both conformations and the feasibility of their interconversion.

Conclusions:

  • Aromatic sheets represent a viable and novel motif for designing complex foldamer structures.
  • The designed foldamers exhibit controllable self-assembly into stable dimeric states with distinct conformations.
  • This work provides insights into the design principles for foldamers capable of large, switchable conformational changes.