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Quantification of ocular surface microcirculation by computer assisted video microscopy and diffuse reflectance

Anne Kari Kvernebo1, Takuma Miyamoto2, Ane Hasle Sporastøyl3

  • 1Department of Ophthalmology, Oslo University Hospital HF, Postboks 4950 Nydalen, 0424, Oslo, Norway; Institute of Clinical Medicine, Faculty of Medicine, University of Oslo, P.O. Box 1171 Blindern, 0318, Oslo, Norway; The Circulation Laboratory, Department of Cardiothoracic Surgery, Oslo University Hospital HF, Postboks 4950 Nydalen, 0424, Oslo, Norway.

Experimental Eye Research
|November 6, 2020
PubMed

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Summary

Digital video microscopy and diffuse reflectance spectroscopy non-invasively assessed piglet conjunctival microcirculation. The limbal conjunctiva showed higher functional capillary density, indicating greater oxygen delivery capacity compared to the bulbar conjunctiva.

Area of Science:

  • Ophthalmology
  • Microcirculation Research
  • Veterinary Science

Background:

  • Assessing conjunctival microcirculation is crucial for understanding ocular health.
  • Previous methods for evaluating microcirculation were often invasive.
  • The metabolic differences between limbal and bulbar conjunctiva were not well-established.

Purpose of the Study:

  • To evaluate the applicability of digital video microscopy and diffuse reflectance spectroscopy for non-invasive assessment of limbal and bulbar conjunctival microcirculation in piglets.
  • To compare microvascular structure and function between limbal and bulbar conjunctiva.
  • To investigate potential differences in metabolic activity reflected in microvascular parameters.

Main Methods:

  • Non-invasive digital video microscopy and diffuse reflectance spectroscopy were employed.
Keywords:
August kroghCapillary flow velocityConjunctival microcirculationFunctional capillary densityLimbal microcirculationMicrovascular oxygen saturationODIN concept

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  • Measurements were taken in sleeping piglets at two international study sites (Oslo University Hospital and Cleveland Clinic).
  • Custom software analyzed recordings to quantify functional capillary density, capillary flow velocity, and microvascular oxygen saturation.

    • Main Results:

    • Functional capillary density was significantly higher in the limbus compared to the bulbar conjunctiva at both sites (p < 0.01).
    • Capillary blood flow velocity was higher in the bulbar conjunctiva compared to the limbus (p < 0.01).
    • Microvascular oxygen saturation levels were recorded, with slight variations between study sites.

    Conclusions:

    • Digital video microscopy and diffuse reflectance spectroscopy are effective non-invasive tools for studying conjunctival microcirculation in piglets.
    • The limbal conjunctiva exhibits a greater capacity for oxygen delivery than the bulbar conjunctiva.
    • These findings support the hypothesis of higher metabolic activity in the limbal conjunctiva.