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Neuropilin and tolloid-like 2 regulates the progression of osteosarcoma

Xuepeng Wang1, Zhenyu Bian1, Changju Hou1

  • 1Department of Orthopedics Surgery, Affiliated Hangzhou First People's Hospital, Zhejiang University School of Medicine, Hangzhou, Zhejiang Province 310006, China.

Gene
|November 6, 2020
PubMed

Insights

Neuropilin and tolloid-like 2 (NETO2) acts as an oncogene in osteosarcoma (OS). Upregulated NETO2 promotes OS cell proliferation and invasion by activating the PI3K/AKT pathway, driving cancer progression.

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Neuropilin and tolloid-like 2 (NETO2) expression is altered in various cancers.
  • The specific role of NETO2 in osteosarcoma (OS) pathogenesis is not well understood.

Purpose of the Study:

  • To investigate the function and mechanism of NETO2 in osteosarcoma cells.
  • To determine if NETO2 acts as an oncogene in osteosarcoma.

Main Methods:

  • Assessed NETO2 expression in human OS samples and cell lines using GEPIA database, mRNA, and protein analysis.
  • Manipulated NETO2 levels (overexpression and downregulation) in OS cells to evaluate effects on proliferation, invasion, migration, colony formation, and epithelial-mesenchymal transition (EMT).
  • Investigated the involvement of the PI3K/AKT signaling pathway and utilized an AKT inhibitor to confirm pathway activation.

Main Results:

  • NETO2 expression was significantly upregulated in human osteosarcoma tissues and cell lines.
  • NETO2 overexpression enhanced OS cell proliferation, invasion, migration, colony formation, and EMT.
  • NETO2 promoted osteosarcoma progression by activating the PI3K/AKT signaling pathway, as evidenced by increased signaling and attenuation of NETO2 effects with an AKT inhibitor.

Conclusions:

  • NETO2 functions as an oncogene in osteosarcoma.
  • NETO2 promotes osteosarcoma development and progression through activation of the PI3K/AKT signaling pathway.
Keywords:
NETO2OsteosarcomaPI3K/AKT

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