Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Confocal Fluorescence Microscopy01:16

Confocal Fluorescence Microscopy

19.4K
Confocal microscopy is an advanced microscopic technique. The prime advantage of the confocal microscope over other microscopy techniques is its ability to block the out-of-focus light from the illuminated samples using pinholes. It is widely used with fluorescence optics to obtain high-resolution, sharp contrast images. Unlike optical microscopes, confocal microscopes use a focused beam of light laser to scan the entire sample surface at different z-planes. These microscopes are, therefore,...
19.4K
Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

603
Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
603
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

11.5K
Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
11.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cortical activity during narrative discourse production in individuals with post-stroke aphasia and controls measured via functional near-infrared spectroscopy.

medRxiv : the preprint server for health sciences·2026
Same author

Visual gamma stimulation causes prolonged enhancement of low-frequency blood flow oscillations across cortical regions in mice.

bioRxiv : the preprint server for biology·2026
Same author

Optical methods for cuffless blood pressure measurements.

Biophotonics discovery·2026
Same author

Comparative validation of speckle contrast optical spectroscopy against diffuse correlation spectroscopy for monitoring human cerebral blood flow.

Neurophotonics·2026
Same author

Mapping Slow Speckle Dynamics to Probe Cellular Metabolic Activity In Vivo using Laser Speckle Contrast Imaging.

bioRxiv : the preprint server for biology·2026
Same author

The neurovascular impulse response function differentially reflects intrinsic neuromodulation across cortical regions.

Nature neuroscience·2026

Related Experiment Video

Updated: Dec 1, 2025

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

14.7K

Dynamic light scattering imaging.

Dmitry D Postnov1,2, Jianbo Tang1, Sefik Evren Erdener1

  • 1Neurophotonics Center, Boston University, Boston, MA 02215, USA.

Science Advances
|November 7, 2020
PubMed
Summary
This summary is machine-generated.

Dynamic light scattering imaging (DLSI) quantifies scatterer dynamics by analyzing speckle fluctuations. This new method improves upon traditional laser Doppler flowmetry and laser speckle imaging for cerebral blood flow analysis.

More Related Videos

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

13.8K
Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts
05:58

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts

Published on: March 29, 2024

1.3K

Related Experiment Videos

Last Updated: Dec 1, 2025

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy
09:16

Measurement of Particle Size Distribution in Turbid Solutions by Dynamic Light Scattering Microscopy

Published on: January 9, 2017

14.7K
Measuring Spatially- and Directionally-varying Light Scattering from Biological Material
11:57

Measuring Spatially- and Directionally-varying Light Scattering from Biological Material

Published on: May 20, 2013

13.8K
Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts
05:58

Author Spotlight: Customized Light-Sheet Imaging for Investigating Myocardial Structures in Rodent Hearts

Published on: March 29, 2024

1.3K

Area of Science:

  • Biomedical optics
  • Photonics
  • Medical imaging

Background:

  • Laser Doppler flowmetry and laser speckle contrast imaging are widely used for cerebral blood flow monitoring.
  • These techniques rely on analyzing speckle patterns generated by scattered light.
  • Limitations exist in their traditional theoretical frameworks for accurately quantifying blood flow dynamics.

Purpose of the Study:

  • To introduce dynamic light scattering imaging (DLSI) for wide-field measurement of speckle temporal intensity autocorrelation.
  • To develop a comprehensive model for identifying dynamic scattering regimes and quantifying scatterer dynamics.
  • To address limitations in existing cerebral blood flow imaging techniques.

Main Methods:

  • Utilizing full temporal sampling of speckle fluctuations.
  • Implementing a comprehensive dynamic light scattering model.
  • Wide-field imaging acquisition.

Main Results:

  • DLSI enables quantitative imaging of scatterer dynamics.
  • Identified errors in the traditional theories of laser Doppler flowmetry and laser speckle contrast imaging.
  • Demonstrated the capability to determine the appropriate dynamic scattering model for cerebral blood flow imaging.

Conclusions:

  • DLSI offers a more accurate approach to measuring cerebral blood flow dynamics.
  • The findings necessitate a re-evaluation of current laser Doppler and laser speckle imaging methodologies.
  • DLSI provides crucial guidance for selecting optimal models in hemodynamic monitoring.