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Visualizing and Isolating Iron-Reducing Microorganisms at the Single-Cell Level.

Cuifen Gan1, Rongrong Wu1, Yeshen Luo1

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Researchers developed a novel fluorescent probe to visualize iron-reducing microorganisms (FeRM). This method allows for the identification and isolation of FeRM from complex samples, advancing environmental microbiology and geochemistry.

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Area of Science:

  • Environmental Microbiology
  • Geochemistry
  • Biotechnology

Background:

  • Iron-reducing microorganisms (FeRM) are crucial in natural and engineered environments.
  • Understanding the in situ location and geochemical functions of FeRM requires effective visualization and isolation techniques.
  • Existing methods are insufficient for differentiating and isolating FeRM from multispecies samples.

Purpose of the Study:

  • To develop a sensitive and selective method for visualizing and isolating active FeRM.
  • To establish a tool for evaluating FeRM activity at the single-cell level.
  • To facilitate the study of FeRM in complex environmental matrices.

Main Methods:

  • Development of a "turn-on" Fe2+-specific fluorescent chemodosimeter (FSFC).
  • Application of FSFC for visualizing FeRM in pure cultures, cocultures, and sediment samples.
  • Integration of FSFC with a single-cell sorter for FeRM isolation and activity verification.

Main Results:

  • The FSFC demonstrated high sensitivity, selectivity, and stability in identifying active FeRM.
  • Fluorescent intensity of FSFC correlated with Fe2+ concentration in bacterial cultures.
  • Successful isolation of three iron-reducing FeRM from an enriched consortium using FSFC and a single-cell sorter.

Conclusions:

  • The developed FSFC is a powerful tool for visualizing and quantifying FeRM activity.
  • This method enables the isolation of FeRM from complex environmental samples.
  • The approach significantly advances the study of FeRM in environmental microbiology, geochemistry, and related fields.