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Related Experiment Video

Updated: Dec 1, 2025

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Comparative Proteomic Analysis of Whole Kidney, Medulla, and Cortical Tubules in Diabetic Pathogenesis of Kidney Injury in Mice

Published on: May 2, 2025

Comparative proteomic analysis identifies biomarkers for renal aging.

Meiqi Yi¹, Yingying Ma¹, Songbiao Zhu¹

¹MOE Key Laboratory of Bioinformatics, Center for Synthetic and Systematic Biology, School of Life Sciences, Tsinghua University, Beijing, China.

|November 7, 2020

Summary

This study identifies 27 potential biomarkers for kidney aging using comparative proteomics. Nicotinamide mononucleotide (NMN) treatment improved kidney peroxisome function in aged mice.

Keywords:

NMN biomarkers glutathionylation proteomics renal aging

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Published on: April 6, 2022

Area of Science:

Gerontology
Proteomics
Molecular Biology

Background:

Proteomics aids in understanding aging molecular signatures.
Inter-dataset validation is crucial for identifying reliable aging biomarkers due to varying methodologies.

Purpose of the Study:

To profile age-associated proteome and glutathionylome changes in mouse kidneys.
To identify potential renal aging biomarkers through comparative analysis.
To investigate the effect of nicotinamide mononucleotide (NMN) on kidney aging.

Main Methods:

Comparative proteomics analysis of young and aged mouse kidneys across three datasets.
Glutathionylome analysis to assess protein modification.
Assessment of NMN administration effects on kidney peroxisomes.

Main Results:

Identified 108 differentially expressed proteins, with 27 potential renal aging biomarkers (e.g., Pck1, Cd5l, Aldh1a1, uromodulin).
Observed downregulation of peroxisomal proteins and upregulation of IgGs in aged kidneys.
Found decreased catalase and glutaredoxin-1 (Glrx1) leading to increased protein glutathionylation.
NMN administration enhanced peroxisome biogenesis in aged mouse kidneys.

Conclusions:

Identified novel potential biomarkers for renal aging.
Peroxisome deterioration is suggested as a hallmark of kidney aging.
NMN shows potential benefits for reducing kidney injury by enhancing peroxisome function.