Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Atomic Force Microscopy01:08

Atomic Force Microscopy

4.0K
Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
4.0K
Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

12.0K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
12.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

HSV-1 origin binding protein UL9 forms intermolecular DNA tethers and intramolecular DNA loops.

Nature communications·2026
Same author

i-Motif, not G-quadruplex, stability regulates insulin expression.

Nucleic acids research·2026
Same author

The transitional kinetics between open and closed Rep structures can be tuned by salt via two intermediate states.

Nucleic acids research·2026
Same author

Structural basis of multitasking by the apicoplast DNA polymerase from Plasmodium falciparum.

Nucleic acids research·2025
Same author

Serial-femtosecond crystallography reveals how a phytochrome variant couples chromophore and protein structural changes.

Science advances·2025
Same author

Structural basis of multitasking by the apicoplast DNA polymerase from <i>Plasmodium falciparum</i>.

bioRxiv : the preprint server for biology·2025

Related Experiment Video

Updated: Dec 1, 2025

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
07:12

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Published on: July 5, 2021

3.8K

The smfBox is an open-source platform for single-molecule FRET.

Benjamin Ambrose1, James M Baxter1, John Cully1

  • 1Sheffield Institute for Nucleic Acids, Department of Chemistry, University of Sheffield, Sheffield, UK.

Nature Communications
|November 7, 2020
PubMed
Summary

We developed the smfBox, an affordable confocal single-molecule Förster Resonance Energy Transfer (smFRET) system. This open-source platform makes advanced biomolecular distance measurements accessible to more researchers.

More Related Videos

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.5K
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.9K

Related Experiment Videos

Last Updated: Dec 1, 2025

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox
07:12

Making Precise and Accurate Single-Molecule FRET Measurements using the Open-Source smfBox

Published on: July 5, 2021

3.8K
FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors
10:34

FRET Microscopy for Real-time Monitoring of Signaling Events in Live Cells Using Unimolecular Biosensors

Published on: August 20, 2012

23.5K
Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes
08:26

Automated Two-dimensional Spatiotemporal Analysis of Mobile Single-molecule FRET Probes

Published on: November 23, 2021

2.9K

Area of Science:

  • Biophysics
  • Biochemistry
  • Molecular Biology

Background:

  • Single-molecule Förster Resonance Energy Transfer (smFRET) measures distances in dynamic biomolecules.
  • High costs and proprietary software limit accessibility of smFRET technology.

Purpose of the Study:

  • To develop a cost-effective and open-source confocal smFRET platform.
  • To democratize the application of smFRET for broader scientific use.

Main Methods:

  • Construction of a confocal smFRET instrument using readily available components.
  • Development of open-source hardware and acquisition software for the smFRET platform.
  • Validation of the smfBox performance through rigorous testing.

Main Results:

  • The smfBox provides a low-cost alternative to commercial smFRET systems.
  • Detailed build instructions and open-source software enable widespread adoption.
  • The platform is fully validated for accurate distance measurements.

Conclusions:

  • The smfBox significantly lowers the barrier to entry for smFRET experiments.
  • This democratized approach empowers non-specialists to utilize smFRET for biomolecular research.
  • The open-source nature fosters community development and innovation in smFRET techniques.