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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

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Updated: Dec 1, 2025

Plunge Freezing: A Tool for the Ultrastructural and Immunolocalization Studies of Suspension Cells in Transmission Electron Microscopy

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Published on: May 5, 2017

Method for cryopreservation of Paramoeba perurans.

Christiane Tröße¹, Mats Kindt¹, Steffen Blindheim²

¹Department of Biological Sciences, University of Bergen, Bergen, Norway.

Journal of Fish Diseases

|November 7, 2020

Summary

A new cryopreservation method enables long-term storage for the aquaculture parasite Paramoeba perurans, which causes amoebic gill disease (AGD). This breakthrough offers a vital tool for maintaining parasite cultures for research and disease management.

Keywords:

Neoparamoeba Paramoeba perurans cryopreservation

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Area of Science:

Aquaculture
Parasitology
Cryobiology

Background:

Amoebic gill disease (AGD), caused by Paramoeba perurans, poses a significant threat to global aquaculture.
Current in vitro culture methods for P. perurans lack long-term storage solutions, hindering research and disease control efforts.

Purpose of the Study:

To develop and evaluate a novel cryopreservation technique for Paramoeba perurans clones.
To assess the viability and success rate of cryopreserved P. perurans following thawing.

Main Methods:

Established a cryopreservation protocol for Paramoeba perurans.
Tested the protocol on five different P. perurans clones.
Evaluated thawing success rates and factors influencing survival.

Main Results:

Successfully cryopreserved four out of five tested P. perurans clones.
Thawing success rates varied among clones, ranging from 25% to 100%.
Older P. perurans clones demonstrated a reduced ability to survive cryopreservation.

Conclusions:

The developed cryopreservation method is effective for long-term storage of most Paramoeba perurans clones.
Clone age is a critical factor affecting cryopreservation success in P. perurans.
This method provides a valuable resource for aquaculture disease management and research.